Finnigan ltq ft ultra mass spectrometer

Full-length protein measurements were performed as described previously (Hoegl et al., 2018 (link)). Proteins were measured on a MassPREP On-Line Desalting Cartridge (Waters) on an Ultimate 3000 HPLC system (Dionex) coupled to a Finnigan LTQ-FT Ultra mass spectrometer (Thermo Fisher Scientific) with electrospray ionization (spray voltage 4.0 kV, tube lens 110 V, capillary voltage 48 V, sheath gas 60 arb, aux gas 10 arb, sweep gas 0.2 arb). Protein Deconvolution Software (Thermo Fisher Scientific, Cat# IQLAAEGABSFANOMBAQ) was used for data analysis and deconvolution.

Intact protein
MS of recombinant protein or proteins modified via the AMPylation assay were performed as described previously.^{45 (link)} The samples were measured with an UltiMate 3000
HPLC system (Dionex) being equipped with a Massprep online desalting
cartridge (Waters) and coupled to a Finnigan LTQ-FT Ultra mass spectrometer
(Thermo Fisher Scientific). Ions were generated by electrospray ionization
(capillary temperature 275 °C, spray voltage 4.0 kV, tube lens
110 V, capillary voltage 48 V, sheath gas 60 arb, aux gas 10 arb,
sweep gas 0.2 arb) and separated with a flow rate of 0.4 mL/min and
the following gradient: (buffer A: 0.1% FA in H₂O, buffer
B: 0.1% FA, 9.9% H₂O in acetonitrile, 1 min hold 6% buffer
B, in 1.5 min increase buffer B to 95%, hold at 95% buffer B for 2
min, decrease to 6% buffer B in 0.2 min, stay at 6% buffer B for further
0.3 min). The instrumented was operated with a resolution of R = 200 000 and a mass range of m/z from 600 to 2000 while acquiring full scans.
The ProMass software for Xcalibur (Version 2.8) was used for deconvolution
(input m/z range: 600–2000;
output mass range: 15 000–30 000 Da).