We measured cell proliferation using a BrdU cell proliferation assay kit (MilliporeSigma, Burlington, MA). Cells were incubated with BrdU for 1 h at 37 °C following incubation for 24 h. Cells were fixed and BrdU incorporation was detected with anti-BrdU detector antibody. The signal was measured using a Ceres UV900H Di ELISA plate reader (Bio-Tek Instruments, Inc., Highland Park, VT). The experiments were completed in triplicate and an average and standard deviation calculated.
Ceres uv900h di elisa plate reader
Manufactured by Agilent Technologies
The Ceres UV900H Di ELISA plate reader is a laboratory instrument designed for performing enzyme-linked immunosorbent assay (ELISA) experiments. It is capable of reading absorbance in 96-well and 384-well microplates using ultraviolet (UV) and visible light wavelengths.
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Lab products found in correlation
2 protocols using ceres uv900h di elisa plate reader
1
BrdU Assay for Cell Proliferation
2
MTT Cell Viability Assay
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We added 10 μl of MTT reagent (5 mg/ml MTT in 1X PBS) to 0.1 ml culture media containing the cells. The mixture was incubated for 3 h at 37 °C, then we added 0.1 ml of solubilization solution (10% SDS in 0.01 M HCl) and incubated the mixture overnight at 37 °C. Absorbances at 570 and 650 nm were measured using a Ceres UV900H Di ELISA plate reader (Bio-Tek Instruments, Inc., Highland Park, VT). Background readings at 650 nm were subtracted from optical density readings at 570 nm. The experiments were completed in triplicate and an average and standard deviation calculated.
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