The breast cancer cell line MCF-7 purchased from American Type Cell Culture, Manassas, Virginia, USA. The materials used for cell culture were from Sigma Aldrich, St. Louis, Missouri, USA. CellTiter® 96 AQueous non-radioactive cell proliferation assay (MTS assay) kit, annexin V-fluorescein isothiocyante (FITC) apoptosis detection kit and DeadEnd™ fluorometric apoptosis detection kit purchased from Promega, Madison, Wisconsin, USA. NE-PER nuclear and cytoplasmic extraction reagents purchased from Thermo Scientific, USA. Mouse monoclonal IgG1 primary antibodies (Bax, Bcl-2, caspase-8, caspase-9, and caspase-3) and anti-mouse m-IgGk BP-HRP secondary antibody purchased from Santa Cruz Biotechnology, Inc., Heidelberg, Germany. All the standard compounds used purchased from Permula, USA. All chemicals used were of analytical grade.
Deadend fluorometric apoptosis detection kit
Manufactured by Promega
Sourced in United States
The DeadEnd™ Fluorometric Apoptosis Detection Kit is a lab equipment product designed to detect and quantify apoptosis (programmed cell death) in cultured cells and tissue sections. It utilizes terminal deoxynucleotidyl transferase (TdT) to label DNA strand breaks, allowing the identification and measurement of apoptotic cells.
Automatically generated - may contain errors
2 protocols using deadend fluorometric apoptosis detection kit
1
Breast Cancer Cell Line MCF-7 Proliferation Assay
2
Quantifying Apoptosis in Kidney Tissue
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Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed to detect cell apoptosis, and sections were stained using the DeadEnd™ Fluorometric Apoptosis Detection Kit (Promega, Madison, WI, USA) according to the manufacturer's instructions. The number of TUNEL-positive tubular cells was counted under an upright fluorescence microscope at ×400 magnification.
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