At the end of the experiment, the recovered cell culture supernatants were used to quantify the concentrations of soluble VCAM-1 (Cat# EK0537, BosterBio), E-selectin (Cat# MBS355367, MyBioSource) and VEGF (Cat# V3-200-820-VEF, Vinci-Biochem). The analysis was performed using ELISA kits according to the manufacturer’s instruction. The analyses were conducted in quadruplicate and the results derived from three independent experiments.
E selectin
Manufactured by MyBioSource
Sourced in United States
E-selectin is a cell adhesion molecule that mediates the initial attachment of leukocytes to the vascular endothelium during the inflammatory response. It plays a crucial role in the recruitment of leukocytes to sites of inflammation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using e selectin
1
Quantifying Soluble Adhesion Molecules
2
Cardiometabolic Biomarkers in Children
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Glucose, insulin, total cholesterol, low density lipoprotein-cholesterol (LDL-C), high density lipoproteincholesterol (HDL-C), triglycerides, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transferase were assayed on children's blood samples after overnight fast. Insulin resistance was calculated by the homeostasis model assessment of insulin resistance (HOMA-IR) [33] . All analyses were performed on COBAS 6000 (Roche Diagnostics, Risch-Rotkreuz, Switzerland). E-Selectin (MyBiosource, San Diego, USA) and LPS (HycultBiotech, Plymouth Meeting, PA, USA) measurement were performed by commercial ELISA kit following the manufacturer's instructions.
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