Horseradish peroxidase hrp conjugated goat anti mouse igg and anti rabbit igg antibodies

Mosquito Aedes albopictus C6/36 cells were maintained at 28°C in Leibovitz medium (L15) supplemented with 10% heat-inactivated fetal bovine serum (FBS). Vero cells and were maintained at 37°C in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% FBS. BHK-21, SK-N-SH, and HEK-293T cells were maintained at 37°C in DMEM supplemented with 10% FBS. Highly purified mouse anti-pan Flavivirus E monoclonal antibody (mAb) 4G2 was produced by RD Biotech (Besançon, France). Mouse mAb anti-JEV NS5 was kindly provided by Y. Matsuura [26 (link)]. Antibodies against Calnexin and SNAP-tag were purchased from Enzo Life Sciences and New England Biolabs, respectively. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and anti-rabbit IgG antibodies were obtained from Bio-Rad Laboratories. HRP-conjugated goat anti-pig antibody was obtained from Bethyl Laboratories. Alexa Fluor 488-conjugated goat anti-mouse IgG antibody was obtained from Jackson ImmunoResearch.

Mouse monoclonal antibody 9E10 anti-HCV NS5A was kindly provided by Charles Rice^{38 (link)} and diluted 1:1000 in TCID₅₀ assays. Commercial antibodies used in Western blotting, along with their respective dilutions, are listed as follow. Mouse monoclonal antibody anti-HCV NS3 was purchased from Abcam (ab65407, 1:3,000). Mouse monoclonal antibody against GAPDH, rabbit polyclonal antibody against CRBN, and rabbit polyclonal antibody against SOD1 were respectively purchased from GeneTex (GTX28245, 1:5,000), Novus Biologicals (NBP1-91810, 1:500), and Sigma-Aldrich (HPA00140-1; 1:500). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and anti-rabbit IgG antibodies were obtained from Bio-Rad Laboratories (170-6516 and 170-6515, respectively, 1:3,000). IRDye® 800CW-conjugated goat anti-rabbit antibody was purchased from LI-COR (926-32211, 1:10,000).

The following commercial antibodies were used: rabbit polyclonal anti-calnexin, anti-PLIN3/TIP47/M6PRBP1 and anti-GFP antibodies from Abcam (Cambridge, UK), and anti-calreticulin antibody from Merck Millipore (Darmstadt, Germany); mouse monoclonal anti-β-tubulin (clone 2-28-33) and anti-c-myc (clone 9E10) antibodies from Sigma (St Louis, MO), anti-Rab8 (clone 4, Rab8), anti-Rab11 (clone 47, Rab11) and anti-GM130 (clone 35, GM130) antibodies from BD Biosciences (Franklin Lakes, NJ), anti-COX IV (clone 1D6E1A8) and anti-transferrin receptor (clone H68.4) antibodies from Life Technologies, anti-β-actin antibody (clone AC-15, Abcam), and anti-PLIN2/ADRP antibody (clone AP 125, PROGEN Biotechnik, Heidelberg, Germany).
The secondary reagents for western blotting were horseradish peroxidase (HRP)-conjugated goat anti-mouse-IgG and anti-rabbit-IgG antibodies (Bio-Rad, Hercules, CA) for analysis of extracts, and HRP–Protein-A (Life Technologies) for immunoprecipitations. The secondary antibodies for immunofluorescence were Alexa Fluor 488, 546 or 647 conjugates of goat anti-mouse-IgG and anti-rabbit-IgG antibodies (Life Technologies).
The following reagents were used: Nile Red, Nocodazole, DAPI and Oil Red O from Sigma; and BODIPY 493/503, BODIPY 558/568 C₁₂ and HCS LipidTOX Red from Life Technologies. Unless otherwise stated, cell culture products were from Life Technologies.