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2 protocols using vinyl stearate

1

Synthesis and Characterization of Carbohydrate Derivatives

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α-D-glucose, maltodextrin, α-CD, vinyl pivalate, vinyl benzoate, pyrene, and dimethylsulfoxide (DMSO) were purchased from Sigma Aldrich. β-CD, γ-CD, vinyl laurate, and vinyl stearate were obtained from Tokyo Chemical Industry Co., Ltd. N, N-dimethylformamide (DMF, anhydrous 99.8%) was obtained from Alfa Aesar, a Johnson Matthey Company. Organic solvents such as methanol and ethanol were of analytical reagent grade, and the water used was triply distilled. Thin-layer chromatography (TLC) was carried out on Merck Kieselgel 60 F254 analytical plates with the specified solvent system. The detection and visualization are followed by spraying 5% sulfuric acid-ethanol and heating for 2 min at 170 °C.
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2

Enzymatic Synthesis of Fatty Acid Esters

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Raffinose (99.0%), t-BuOH (anhydrous), pyridine (anhydrous), N, N-dimethylformamide (DMF), benzene, Span 20, Span 40, Span 60, Span 80, Tween 20 and Tween 80 were purchased from the Energy Chemical Co., Ltd (Shanghai, China). Vinyl hexanoate (>99.0%), vinyl n-octanoate (>99.0%), vinyl decanoate (>99.0%), vinyl laurate (>99.0%), vinyl myristate (>99.0%), vinyl palmitate (>96.0%) and vinyl stearate (>95.0%) were purchased from the Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Novo Nordisk (Bagsvaerd, Denmark) provided lipozyme TLIM while the Mitsubishi-Chemical Foods Corporation was the source of the sucrose esters P-1570 and P-1670. Pure olive oil, on the other hand, was purchased from a local food store.
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