Ccr9 242503

Manufactured by R&D Systems

CCR9 (242503) is a recombinant human chemokine receptor protein. It is a G protein-coupled receptor that binds to the chemokine TECK/CCL25 and is involved in the trafficking and homing of lymphocytes.

Automatically generated - may contain errors

Lab products found in correlation

Facsdiva, by BD (1 mentions) Cd16 cd32 2.4g2, by BD (1 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) I ab af6 1201, by BD (1 mentions) Il 17a tc11 18h10.1, by BioLegend (1 mentions) Ccr7 4b12, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

PE-labeled CCR9 (242503) (2 citations)

2 protocols using ccr9 242503

Multiparameter Flow Cytometry Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

iIEC and intraluminal cells (iLC) were identified by immunostaining with following fluorochrome-conjugated antibodies: CD3e (145-2C11), B220 (RA36B2), class II (M5/114.15.2), CD11b (M1/70), CD11c (N418), PDCA-1 (eBio927), CD45.1 (A20) and CD45.2 (104) from eBiosciences; Ly6C (AL-21) and CD103 (M290) from BD Biosciences; CCR9 (242503) from R&D Systems. Unfixed cells were analyzed using an LSR II (BD Biosciences) to collect raw cytometric data. Off-line analyses of cell populations were performed using either FACS Diva (BD Biosciences) or FlowJo software (Tree Star). Instrument calibration, construction of off-line compensation matrices to discriminate between background and true fluorescence signals, and electronic gating of cell populations of interest followed previously established procedures [29 (link), 30 (link)].

Hoffman R.A., Huang S., Chalasani G, & Vallejo A.N. (2021). Disparate Recruitment and Retention of Plasmacytoid Dendritic Cells to The Small Intestinal Mucosa between Young and Aged Mice. Aging and Disease, 12(5), 1183-1196.

+ Open protocol

+ Expand

Integrin-Mediated Immune Cell Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

The anti-integrin β1 monoclonal antibody (mAb), KMI6, was purchased from OriGene Technologies (Rockville, MD). Blocking mAbs to integrin α1 (clone HMα1), α4 (R1-2), α5 (5H10-27), β1 (HMβ1-1), and β7 (FIB27) were from BioLegend (San Diego, CA); αE/CD103 (M290) and β1 (9EG7) were from BD Biosciences (San Jose, CA). The following mAbs were used for flow cytometry and cell sorting: CD11c (N418), CD40 (1C10), CD86 (GL1), CD103 (2E7), integrin α1 (HMα1), α2 (HMα2), α4 (R1-2), α5 (5H10-27), α6 (GoH3), αV (RMV-7), β1 (HMβ1-1), β7 (FIB504), α4β7 (DATK32), IFN-γ (XMG1.2), and IL-17A (TC11-18H10.1) were from BioLegend; E-cadherin (114420) and CCR9 (242503) were from R&D Systems; CCR7 (4B12) and Foxp3 (FJK-16s) were from eBioscience (San Diego, CA); CD16/CD32 (2.4G2) and I-A^b (AF6-120.1) were from BD Biosciences.

Yokota-Nakatsuma A., Ohoka Y., Takeuchi H., Song S.Y, & Iwata M. (2016). Beta 1-integrin ligation and TLR ligation enhance GM-CSF–induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties. Scientific Reports, 6, 37914.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!