Ppackh1 packaging plasmid

Manufactured by System Biosciences

The PPACKH1 packaging plasmid is a laboratory tool designed for the production of lentiviral particles. It contains the necessary viral packaging genes required for the assembly of lentiviral particles in a cell line. The plasmid provides the structural and enzymatic components needed for viral particle formation, without the inclusion of the viral genome. This allows for the generation of lentiviral particles that can be used for various research applications, such as gene delivery and gene expression studies.

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Lab products found in correlation

Pcdh ef1 mcs bgh pgk gfp t2a puro vector, by System Biosciences (1 mentions) Puromycin, by Merck Group (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PPACKH1 packaging plasmid mix (19 citations) PPACKH1 (6 citations) PPACKH1 Lentivector Packaging Plasmid mix (6 citations)

2 protocols using ppackh1 packaging plasmid

Generating Lentiviral CDA Overexpression

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Full-length cDNA of human CDA cDNA [22 (link)] was subcloned into pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro Vector (System Bioscience) to generate a pCDH-CDA construct. Lentiviral particles were produced by co-transfection with pCDH-CDA and pPACKH1 packaging plasmid (System Bioscience) into 293TN cells using Lipofectamine LTX, in accordance with the manufacturer’s instructions. MDS-L overexpressing CDA (MDS-L/CDA) cells were then established by lentiviral infection.

Murakami Y., Kimura Y., Kawahara A., Mitsuyasu S., Miyake H., Tohyama K., Endo Y., Yoshida N., Imamura Y., Watari K., Ono M., Okamura T, & Kuwano M. (2019). The augmented expression of the cytidine deaminase gene by 5-azacytidine predicts therapeutic efficacy in myelodysplastic syndromes. Oncotarget, 10(23), 2270-2281.

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Overexpression of TPPP in Pancreatic Cancer Cells

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TPPP-overexpressing lentiviruses were constructed by Hanbio Biotechnology Co., Ltd. (Shanghai, China). The full-length coding region of human TPPP was sub-cloned into the HBLV-h-TPPP-3 × FLAG-GFP-PURO plasmid (System Biosciences, Mountain View, CA, USA). The verified recombinant vector and the pPACKH1 packaging plasmid (System Biosciences) were co-transfected into 293T cells using Lipofectamine 3000 reagent (Life Technologies). The supernatant of the cultured 293T cells was collected to infect BxPC-3 cells, BxPC-3-YY1 cells, PANC-1 cells and PANC-1-YY1 cells. The pHBLV-CMV-MCS-3 × FLAG-EF1-ZsGreen-T2A-PURO vector was used to package the virus and infect BxPC-3 cells, BxPC-3-YY1 cells, PANC-1 cells and PANC-1-YY1 cells as a control. Stable cell lines were selected by culturing in medium containing 5 μg/ml puromycin (Sigma). TPPP expression was confirmed by qRT-PCR and western blot.

Chen Q., Yang C., Chen L., Zhang J.J., Ge W.L., Yuan H., Meng L.D., Huang X.M., Shen P., Miao Y, & Jiang K.R. (2019). YY1 targets tubulin polymerisation-promoting protein to inhibit migration, invasion and angiogenesis in pancreatic cancer via p38/MAPK and PI3K/AKT pathways. British Journal of Cancer, 121(11), 912-921.

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