The EPCs-Exos were labeled using PKH67 kits (Sigma-Aldrich). Exos were incubated with PKH67 membrane dye (4 μL, Sigma-Aldrich) and diluent C (1 mL) for 4 min. The labeled Exos were filtered using the Exoquick Exo precipitation solution and suspended in the basic medium. HUVECs were incubated with the above solution (250 μL) for 3 h. Then, the samples were incubated with 4% paraformaldehyde (1 mL) for 0.5 h. The nucleus was stained with 4’,6-diamidino-2-phenylindole (DAPI). The images were observed using a fluorescence microscope (Zeiss, LSM700B, Germany).
Pkh 67 kits
Manufactured by Merck Group
Sourced in United States
PKH-67 kits are a type of laboratory equipment used for fluorescent cell labeling. The kits contain a green fluorescent dye that can be used to stain cells, allowing for their visualization and tracking in various experimental setups. The core function of these kits is to provide a tool for researchers to label and study cells using fluorescence microscopy or flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Actingreentm 488, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 568, by Proteintech (2 mentions)
Cdc42 inhibitor ml141, by MedChemExpress (2 mentions)
Lipofectaminetm rnaimax, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488, by Proteintech (2 mentions)
Fmtm 4 64fx, by Thermo Fisher Scientific (2 mentions)
Anti ki67, by Cell Signaling Technology (2 mentions)
Calnexin, by Abcam (2 mentions)
Cdc42, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Anti cd9, by Abcam (2 mentions)
Pkh67 membrane dye, by Merck Group (1 mentions)
Lsm700b, by Zeiss (1 mentions)
Cd105, by Abcam (1 mentions)
Pkh26, by Merck Group (1 mentions)
3 protocols using pkh 67 kits
1
Visualizing Exosome Uptake by HUVECs
2
Immunofluorescence analysis of Cdc42 in cells
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Anti-CD9, CD63, Alix, calnexin, CD31, CD34, CD45, CD73, CD90 and CD105 antibodies were purchased from Abcam (Cambridge, UK). Anti-Ki-67, RhoA, Rac1, Cdc42 and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, USA). Alexa Fluor 488 and Alexa Fluor 568 secondary antibodies were purchased from Proteintech (Rosemont, IL, USA). Goat anti-rabbit/anti-mouse IgG IRDyel 800cw secondary antibodies were purchased from Abbkine (Redlands, CA, USA). PKH-26 and PKH-67 kits were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine TM RNAiMAX, FM TM 4-64FX and ActinGreen TM 488 were purchased from Thermo Fisher (Eugene, Dregon, USA). SiCdc42, Cdc42-EGFP and Cdc42-mCherry fusion protein expression plasmids were purchased from GenePharma (Suzhou, China). Cdc42 inhibitor ML141 was purchased from MedChemExpress (Monmouth Junction, USA).
3
Characterization of Cdc42 Regulators
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Anti-CD9, CD63, Alix, calnexin, and CD31 antibodies were purchased from Abcam (Cambridge, UK). Anti-Ki-67, RhoA, Rac1, Cdc42 and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, USA). Alexa Fluor 488 and Alexa Fluor 568 secondary antibodies were purchased from Proteintech (Rosemont, IL, USA). Goat anti-rabbit/anti-mouse IgG IRDyel 800cw secondary antibodies were purchased from Abbkine (Redlands, CA, USA). PKH-67 kits were purchased from Sigma-Aldrich (St.
Louis, MO, USA). Lipofectamine TM RNAiMAX, FM TM 4-64FX and ActinGreen TM 488 were purchased from Thermo Fisher (Eugene, Dregon, USA). SiCdc42, Cdc42-EGFP and Cdc42-mCherry fusion protein expression plasmids were purchased from GenePharma (Suzhou, China). Cdc42 inhibitor ML141 was purchased from MedChemExpress (Monmouth Junction, USA).
Louis, MO, USA). Lipofectamine TM RNAiMAX, FM TM 4-64FX and ActinGreen TM 488 were purchased from Thermo Fisher (Eugene, Dregon, USA). SiCdc42, Cdc42-EGFP and Cdc42-mCherry fusion protein expression plasmids were purchased from GenePharma (Suzhou, China). Cdc42 inhibitor ML141 was purchased from MedChemExpress (Monmouth Junction, USA).
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