Abbit anti iba 1

To examine the cell type expressing PPARγ immunoreactivity, the sections after the ischemic surgery were processed by double immunofluorescence staining. Double immunofluorescence staining was performed using mouse anti-PPARγ (1:50, Santa Cruz Biotechnology)/rabbit anti-Iba-1 (1:200, Wako, Nuss, Germany) for microglia or rabbit anti-glial fibrillary acidic protein (GFAP; 1:200, Chemicon International, Temecula, CA, USA) for astrocytes. The sections were incubated in the mixture of antisera overnight at room temperature, and then were incubated in a mixture of both Cy3-conjugated goat anti-mouse IgG (1:200, Jackson ImmunoResearch, West Grove, PA, USA) and FITC-conjugated goat anti-rabbit IgG (1:200, Jackson ImmunoResearch) for 2 hours at room temperature. The immunoreactions were observed under the confocal microscope (LSM510 META NLO, Carl Zeiss).