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Anti cd20 fitc

Manufactured by Beckman Coulter
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The Anti-CD20-FITC is a fluorescently labeled antibody that binds to the CD20 antigen expressed on the surface of B cells. It is used in flow cytometry applications to identify and quantify B cell populations.

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Lab products found in correlation

Anti cd56 apc, by Beckman Coulter (2 mentions) Anti cd8 pacificblue, by Thermo Fisher Scientific (2 mentions) Anti cd11c fitc bu15, by Thermo Fisher Scientific (2 mentions) Anti cd14 apc rmo52, by Beckman Coulter (2 mentions) Anti cd16 pacificblue 3, by Beckman Coulter (2 mentions) Anti cd19 apc, by Beckman Coulter (2 mentions) Anti mica b pe 6d4, by R&D Systems (2 mentions) Anti ulbp1 pe, by R&D Systems (2 mentions) Anti ulbp2 5 6 pe, by R&D Systems (2 mentions) Anti cd4 apc, by Thermo Fisher Scientific (2 mentions) Anti ulbp3 pe, by R&D Systems (2 mentions) Anti cd3 alexa fluor 700 uc ht1, by Thermo Fisher Scientific (2 mentions) Anti cd19 pe, by Beckman Coulter (1 mentions) Anti cd3 apc, by Beckman Coulter (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

3 protocols using anti cd20 fitc

1

Multicolor Flow Cytometry for B and T cells

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PBMC were stained with anti-CD20 FITC (Beckman), anti-CD19 PE (Beckman), and anti-CD3 APC (Beckman); dead cells were labeled with DAPI (Invitrogen). Cytometry gating was performed first on lymphocytes, then live cells (DAPI-negative), then on either CD19+ or CD3+, and lastly on CD20+ among CD19+ or CD3+ cells.
Palanichamy A., Jahn S., Nickles D., Derstine M., Abounasr A., Hauser S.L., Baranzini S.E., Leppert D, & von Büdingen H.C. (2014). Rituximab efficiently depletes increased CD20 expressing T cells in multiple sclerosis patients. Journal of immunology (Baltimore, Md. : 1950), 193(2), 580-586.
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2

Multiparameter Immune Cell Analysis

Cited 1 time
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PBMC staining and analysis were as described [3 (link)]. Unless otherwise specified, mAbs were from BD Pharmingen and included anti-CD3-Alexa Fluor 700 (UC-HT1), anti-CD4-APC (RPA-T4), anti-CD8-PacificBlue (RPA-T8), anti-CD11c-FITC (BU15, Thermo Scientific), anti-CD14-APC (RMO52, Beckman Coulter), anti-CD16-PacificBlue (3G8), anti-CD19-APC (HIB19) and anti-CD20-FITC (L27), anti-CD56-APC (N901, Beckman Coulter), and anti-NKG2D-PE (1D11; [2 (link)]). Anti-NKG2DL mAb were anti-MICA/B-PE (6D4), anti-ULBP1-PE (170818, R & D Systems), anti-ULBP2/5/6-PE (165903; R & D Systems), anti-ULBP3-PE (166510; R & D Systems), and anti-ULBP4-PE (1H1; [12 (link)]). In some cases, viable cell numbers were limiting resulting in partial data sets.
Hamada S., Caballero-Benitez A., Duran K.L., Stevens A.M., Spies T, & Groh V. (2017). Soluble MICB in Plasma and Urine Explains Population Expansions of NKG2D+CD4 T Cells Inpatients with Juvenile-Onset Systemic Lupus Erythematosus. Open journal of immunology, 7(1), 1-17.
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3

Multiparameter Immune Cell Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC staining and analysis were as described [3 (link)]. Unless otherwise specified, mAbs were from BD Pharmingen and included anti-CD3-Alexa Fluor 700 (UC-HT1), anti-CD4-APC (RPA-T4), anti-CD8-PacificBlue (RPA-T8), anti-CD11c-FITC (BU15, Thermo Scientific), anti-CD14-APC (RMO52, Beckman Coulter), anti-CD16-PacificBlue (3G8), anti-CD19-APC (HIB19) and anti-CD20-FITC (L27), anti-CD56-APC (N901, Beckman Coulter), and anti-NKG2D-PE (1D11; [2 (link)]). Anti-NKG2DL mAb were anti-MICA/B-PE (6D4), anti-ULBP1-PE (170818, R & D Systems), anti-ULBP2/5/6-PE (165903; R & D Systems), anti-ULBP3-PE (166510; R & D Systems), and anti-ULBP4-PE (1H1; [12 (link)]). In some cases, viable cell numbers were limiting resulting in partial data sets.
Hamada S., Caballero-Benitez A., Duran K.L., Stevens A.M., Spies T, & Groh V. (2017). Soluble MICB in Plasma and Urine Explains Population Expansions of NKG2D+CD4 T Cells Inpatients with Juvenile-Onset Systemic Lupus Erythematosus. Open journal of immunology, 7(1), 1-17.
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