Alexa fluor 647 conjugated goat anti mouse igg antibodies

Cells were grown on 35-mm matTek dishes (MatTek corporation
#P35G-1.5-14-c) overnight and were induced with 2μg/ml of doxycycline for
24 hours. The cells were fixed with 4% formaldehyde solution for 10 min at room
temperature (RT) and then blocked with protein block serum-free buffer (Dako
#x0909) for 30 min. Subsequently, cells were gently washed with 0.05% NP-40/PBS
3 times and stained with mouse anti-ZIKV E monoclonal antibody (BioFront
BF-1176-56) for 2 hours at RT using Antibody Diluent with Background Reducing
Component (Dako #S3022). The cells were washed three times with 0.05% NP-40/PBS
for 5 minutes and then were incubated with 1:1000 Alexa Fluor™
647-conjugated goat anti-mouse IgG antibodies (Abcam ab150115) for 1 hour at
room temperature. After additional washing, images were captured using a
DeltaVision Elite microscope (Applied Precision). The images were analyzed using
Volocity software (Quorum Technologies, Inc.).