The cell cycle was analyzed by propidium iodide (PI) staining at the indicated time points as previously described (24 (link)). Apoptotic cells were detected by staining with the fixable viability dye eFluor 660 (catalog no. 650864; eBioscience, San Diego, CA) and a phycoerythrin (PE)-Cy7 annexin V apoptosis detection set (catalog no. 88810374; eBioscience) according to the instructions of the manufacturer. Flow cytometry was performed in a FACSCanto system (BD Biosciences, San Jose, CA), and analysis was performed with FlowJo (FlowJo, LLC, Ashland, OR).
Pe cy7 annexin 5 apoptosis detection set
Manufactured by Thermo Fisher Scientific
The PE)-Cy7 annexin V apoptosis detection set is a laboratory product used to detect and measure apoptosis in cells. It contains Annexin V conjugated with the fluorescent dye PE-Cy7, which binds to phosphatidylserine, a marker for apoptotic cells. The set also includes binding buffer and propidium iodide for additional cell analysis.
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Lab products found in correlation
3 protocols using pe cy7 annexin 5 apoptosis detection set
1
Cell Cycle and Apoptosis Analysis
2
Cell Cycle and Apoptosis Analysis
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Cell cycle analysis was performed by propidium iodide (PI) staining, and flow cytometry was carried out with a FACSCanto II flow cytometry system (BD Biosciences). The fixable viability dye eFluor 660 kit (650864; eBioscience) and a phycoerythrin (PE)-Cy7 annexin V apoptosis detection set (88810374; eBioscience) were used to detect apoptotic cells following the instructions of the manufacturer. The data were analyzed with the FlowJo software (BD Biosciences).
3
Cell Cycle Progression and Apoptosis Analysis
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Cell cycle and BrdU incorporation were performed at the indicated time points as previously described[12 (link)]. Cell cycle was analyzed by propidium iodide (PI) staining. BrdU incorporation was performed by pulsing cells with 10 μM BrdU for 1 h and then stained with a Pacific Blue monoclonal antibody to BrdU (Cat. B35129, Life Technologies, Grand Island, NY). Apoptotic cells were detected by Fixable Viability Dye eFluor 660 staining (Cat. 650864, eBioscience, San Diego, CA) and with a PE-Cy7 Annexin V Apoptosis Detection Set (Cat. 88810374, eBioscience) following the instructions of the manufacturer. Flow cytometry was performed in a FACSCanto System (BD Biosciences, San Jose, CA) and analysis was performed with FlowJo (FlowJo, LLC, Ashland, OR).
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