Cells were FACS-sorted on precoated Shandon Cytoslides (#5991051, Thermo Fisher Scientific) and allowed to attach for 30 min. The cells were fixed with 4% formaldehyde, washed in 1x PBS, permeabilized with TBS +0.1% Triton, and incubated overnight with primary antibody. Next, the cells were incubated with a peroxidase-labeled secondary antibody from an ImmPRESS HRP Polymer Detection Kit (MP-7401, Vector Laboratories, Burlingame, CA, USA) per the manufacturer's instructions and developed with liquid DAB (SK4100, Vector Laboratories). The cells were counterstained with hematoxylin, rinsed, and mounted. A list of primary and secondary antibodies is supplied in Supplementary Table 1 .
Shandon cytoslides
Manufactured by Thermo Fisher Scientific
The Shandon Cytoslides are specialized glass slides designed for use in cytological and histological applications. They provide a standardized platform for the preparation and analysis of cellular samples. The slides feature a defined circular area for sample deposition, allowing for consistent and reproducible sample presentation during microscopic examination.
Automatically generated - may contain errors
2 protocols using shandon cytoslides
1
Immunohistochemical Staining Protocol
2
Eosinophil Differential Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were counted and cytospun onto poly-L-Lysine coated slides (Shandon Cytoslides, Thermo Scientific) at 800 rpm for 3 minutes (Shandon Cytospin 2). Eosinophils were then differentiated by cytological staining using a commercial kit (Diff-Quik, Polysciences, Warrington, PA) per the manufacturer’s instructions. Finally, slides were mounted with Entellan Mounting Medium (Electron Microscopy Sciences).
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