Immunoprecipitation was performed as described previously [24 (link)]. Briefly, cell lysates were immunoprecipitated with a p47phox antibody (Santa Cruz Biotechnology, Dallas, TX, USA) overnight at 4 °C. The immunocomplexes were collected by washing six times with lysis buffer, boiled in 1 × Laemmli sample buffer (Bio-Rad) for 10 min and subjected to Western blot analysis with an anti-Nox2 antibody (Abcam).
P47phox antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The P47phox antibody is a laboratory reagent used to detect and study the p47phox subunit of the NADPH oxidase complex. The p47phox protein plays a crucial role in the activation of the NADPH oxidase enzyme, which is involved in the production of reactive oxygen species in various cell types. The antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate the expression and localization of the p47phox protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti nox2 antibody, by Abcam (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
Goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Trypsin, by Beyotime (1 mentions)
Ecl plus kit, by Beyotime (1 mentions)
Superoxide dismutase sod, by Nanjing Jiancheng (1 mentions)
Benzamil, by Merck Group (1 mentions)
Hexamethonium, by Merck Group (1 mentions)
Lucigenin, by Santa Cruz Biotechnology (1 mentions)
Rac1 antibody, by Santa Cruz Biotechnology (1 mentions)
Gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
3 protocols using p47phox antibody
1
Immunoprecipitation and Western Blot Analysis
2
Antioxidant Effects of Pioglitazone
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Dulbecco's modified Eagle medium (DMEM) and 1% penicillin/streptomycin were purchased from HyClone (UT, USA); fetal bovine serum (FBS) was purchased from Hangzhou Sijiqing Biological Engineering Materials Co., Ltd. (Hangzhou, China); trypsin, BCA protein assay kit, and the ECL Plus kit were from Beyotime Institute of Biotechnology (Nantong, China); pioglitazone was a gift from Jiang Su Heng Rui Medicine Co., Ltd. (Lianyungang, China); 2′,7′-dichlorofluorescein diacetate (DCFH-DA) and ethidium bromide (EB) were from Sigma (MO, USA); rabbit p22phox, p47phox antibody and goat anti-rabbit IgG were purchased from Santa Cruz Biotechnology Inc. (CA, USA); β-actin was from Sangon Biological Engineering Technology Corporation (Shanghai, China); TRIzol was obtained from Invitrogen (CA, USA); reverse transcription reagents and pcr kit were from TaKaRa Biotechnology Co., Ltd. (Dalian, China); superoxide dismutase (SOD) and malonaldehyde (MDA) assay were from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
3
DOCA-Induced Hypertension Protocol
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Deoxycorticosterone acetate pellets (DOCA, #SM-121) are provided by Innovative Research of America. Isoflurane is the product of Shenzhen Reward Company. Amlodipine besylate tablets (AML, #H10950224) are produced by Pfizer Pharmaceutical Company. Dried rhizomes of Ligusticum chuanxiong Hort (#801003917, chuanxiong) and Gastrodia elata Bl (#801002511, Tianma) were provided by Beijing TongRenTang (Bozhou) Co. Ltd.., which were identified by Professor Hu Benxiang of School of Pharmacy, Shaanxi University of Chinese Medicine, as specified varieties in Pharmacopoeia. p67phox antibody (#sc-374510), p47phox antibody (#sc-17845), Rac1 antibody (#sc-514583), ENaC antibody (#sc-48428), and GAPDH antibody (#sc-47724) were provided by Santa Cruz Biotechnology Company. Lucigenin (#sc-202698A) is produced by Santa Cruz Biotechnology Company. NE enzyme-linked immunosorbent assay kit (ELISA, #BAE-5200) is provided by Rocky Mountain Diagnostics Company. Benzamil (#B2417) and hexamethonium (#H2138) are provided by Sigma-Aldrich.
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