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Rabbit anti lc3b d11

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Rabbit anti-LC3B (D11) is a primary antibody that recognizes the microtubule-associated protein 1A/1B-light chain 3 (LC3B). LC3B is a widely used marker for the detection of autophagosomes, which are double-membrane vesicles involved in the autophagic process.

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Lab products found in correlation

Glyceryl trioleate, by Merck Group (1 mentions) Imperial protein stain, by Thermo Fisher Scientific (1 mentions) Bodipy 558 568 c12, by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rabbit anti gfp, by Cell Signaling Technology (1 mentions) Expi293 medium, by Thermo Fisher Scientific (1 mentions) Flag m2 resin, by Merck Group (1 mentions) Rabbit anti p62, by Cell Signaling Technology (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Nbd ps, by Avanti Polar Lipids (1 mentions) Liss rhod pe, by Avanti Polar Lipids (1 mentions) Pi 4 5 p2, by Avanti Polar Lipids (1 mentions) Dgs nta ni, by Avanti Polar Lipids (1 mentions) Ab62720, by Abcam (1 mentions) Rabbit anti lc3b ab48394, by Abcam (1 mentions) Horseradish peroxidase hrp conjugated goat anti mouse and goat anti rabbit antibodies, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti iba1 019 19741, by Fujifilm (1 mentions)

3 protocols using rabbit anti lc3b d11

1

Monitoring Autophagy Lipid Dynamics

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DOPE (Avanti Polar Lipids; 850725), DOPC (Avanti Polar Lipids; 850375), NBD-PS (Avanti Polar Lipids; 810198), Liss Rhod-PE (Avanti Polar Lipids; 810150), DGS-NTA(Ni) (Avanti Polar Lipids; 790404), PI4,5P2 (Avanti Polar Lipids; 840046), glyceryl trioleate (Sigma-Aldrich; T7140), BODIPY 558/568 C12 (Invitrogen; D3835), Imperial protein stain (Thermo Scientific; 24617), Flag M2 resin (Sigma-Aldrich; A2220), WIPI-4-TurboGFP (Origene; WDR45-tGFP transcript variant 1, RG209654), BSA-Oleate monounsaturated fatty acid complex and BSA control (Cayman Chemical; 29557 and 29556), rabbit anti-ATG2A (Cell Signalling; 15011), rabbit anti-ATG2B (Sigma-Aldrich; HPA019665), mouse anti-WIPI-4 (Santa Cruz Biotechnology; sc398272), rabbit anti-LC3B (D11) (Cell Signalling; 3868), rabbit anti-p62 (Cell Signalling; 95697), rabbit anti-Plin3 (Sigma-Aldrich; HPA006427), rabbit anti-beta-actin (Cell Signalling; 4970), mouse anti-Flag M2 (Millipore; F1804), rabbit anti-GFP (Cell Signalling; 2956), secondary antibodies, AlexaFluor 647 goat anti-mouse (Life Technologies; A21236), sodium dithionite Sigma-Aldrich (157953), DMEM (Gibco; 11965-092), MEM (Gibco; 31985-070), Expi293 medium (Gibco; A14351-01), 1x EBSS (Gibco; 24010-043).
Korfhage J.L., Wan N., Elhan H., Kauffman L., Pineda M., Fuller D.M., Thiam A.R., Reinisch K.M, & Melia T.J. (2023). ATG2A-mediated bridge-like lipid transport regulates lipid droplet accumulation. bioRxiv.
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2

Immunodetection of Cellular Markers

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Commercially available primary antibodies used were: mouse anti-β-Actin (A2228; Sigma-Aldrich, St. Louis, MO, United States), rabbit anti-LC3A (ab62720), rabbit anti-HMGCS2 (ab137043, EPR8642) rabbit anti-4 Hydroxynonenal (4-HNE) antibody (ab46545) from Abcam (Cambridge, MA, United States), rabbit anti-LC3B (ab48394) from Abcam, rabbit anti-LC3B (D11) from Cell Signaling Technology (Danvers, MA, United States), rabbit anti-Iba1 (019-19741) from Wako Chemicals, Japan. Secondary antibodies used were: goat anti-mouse and goat anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies (Thermo Fisher Scientific), goat anti-mouse and anti-rabbit IgG Alexa Fluor 488/594/647 conjugates (Invitrogen).
Dhingra A., Bell B.A., Peachey N.S., Daniele L.L., Reyes-Reveles J., Sharp R.C., Jun B., Bazan N.G., Sparrow J.R., Kim H.J., Philp N.J, & Boesze-Battaglia K. (2018). Microtubule-Associated Protein 1 Light Chain 3B, (LC3B) Is Necessary to Maintain Lipid-Mediated Homeostasis in the Retinal Pigment Epithelium. Frontiers in Cellular Neuroscience, 12, 351.
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3

Endogenous LC3B Intracellular Staining

Cited 1 time
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Intracellular staining and analysis of endogenous saponin resistant LC3B was performed as previously described [9 (link), 10 (link), 30 (link)] using rabbit anti-LC3B (D11; Cell Signaling) followed by phycoerythrin (PE) conjugated goat anti-rabbit IgG (Santa Cruz Biotechnology).
Campbell G.R., Rawat P., Bruckman R.S, & Spector S.A. (2015). Human Immunodeficiency Virus Type 1 Nef Inhibits Autophagy through Transcription Factor EB Sequestration. PLoS Pathogens, 11(6), e1005018.
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