The RL95-2 (ATCC®CRL-1671™) and HEC-1-A (ATCC®HTB-112™) human endometrial carcinoma cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium nutrient mixture F-12 (DMEM/F12) supplemented with 10% fetal bovine serum (FBS), 0.005 mg/mL insulin, and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). HEC-1-A cells were cultured in McCoy’s 5A medium supplemented with 10% FBS and 1% penicillin–streptomycin. Doxorubicin, cisplatin, sodium palmitate, 2′,7-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI), and thiazolyl blue tetrazlium bromide (MTT) were obtained from Sigma Aldrich (St. Louis, MO, USA).
2 7 dichlorofluorescein diacetate dcfh da propidium iodide
Manufactured by Merck Group
Sourced in United States
2′,7-dichlorofluorescein diacetate (DCFH-DA) is a cell-permeant, non-fluorescent probe that can be used to measure intracellular oxidative stress. Propidium iodide (PI) is a fluorescent intercalating agent that can be used to stain DNA and assess cell viability.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Doxorubicin, by Merck Group (2 mentions)
Rl95 2, by Thermo Fisher Scientific (2 mentions)
Cisplatin, by Merck Group (2 mentions)
Rl95 2 atcc crl 1671, by American Type Culture Collection (2 mentions)
Mitosox red, by Thermo Fisher Scientific (2 mentions)
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Thiazolyl blue tetrazlium bromide mtt, by Merck Group (1 mentions)
Sodium palmitate, by Merck Group (1 mentions)
Hec 1 a atcc htb 112, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
Tramadol, by Merck Group (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Hec 1a, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
3 protocols using 2 7 dichlorofluorescein diacetate dcfh da propidium iodide
1
Endometrial Carcinoma Cell Lines for Cytotoxicity Assays
2
H9c2 Metabolic Stress Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H9c2 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin (Invitrogen; Waltham, MA, USA). Glucose, palmitic acid, 2′,7-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI), and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MitoSOX Red was obtained from Invitrogen.
3
Endometrial Cancer Cell Lines Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human endometrial adenocarcinoma cell lines RL95-2 (ATCC®CRL-1671™) and HEC-1-A cells (ATCC®HTB-112™) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). For the cell culture experiments, we cultured RL95-2 and HEC-1-A cells in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F12) and McCoy’s 5A medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, USA), respectively. Tramadol, doxorubicin, cisplatin, 2′,7-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI), and thiazolyl blue tetrazolium bromide (MTT) were obtained from Sigma Aldrich (Sigma Aldrich; St. Louis, MO, USA). MitoSOX Red was obtained from Invitrogen (Invitrogen; Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!