TZM-bI cells were seeded at 10,000 cells per well in 100 μL medium in 96-well black plates with half area transparent bottom (Greiner Bio-One). Each well was inoculated with 100 μL of viruses that were subjected to 10-fold serial dilution and infection was done in triplicate. After 48 h, viral infection was determined by a Firefly Luciferase Assay Kit (Biotium). Briefly, after removal of media, 20 μL of 1x Firefly Lysis Buffer were added to each well. After 5 m, 15 μL of Firefly Luciferase Assay Buffer 2.0 containing 0.3 μL of D-luciferin at 10 mg/ml were added to each well and luciferase activities were measured by Veritas Microplate luminometer (Turner Biosystem). Viral infectivity was calculated by normalizing luciferase activities with the amounts of p24Gag in viral inoculum.
Half area transparent bottom
Manufactured by Greiner
The Half Area Transparent Bottom is a specialized lab equipment designed to provide a clear, unobstructed view of the lower half of a sample or specimen. This equipment features a transparent bottom surface that allows for easy observation and analysis of the lower portion of the sample, while the upper half remains accessible for manipulation or further processing. The core function of this product is to enable detailed examination and study of the lower regions of a sample in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using half area transparent bottom
1
Viral Infectivity Quantification Assay
2
Viral Infectivity Quantification Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TZM-bI cells were seeded at 10,000 cells per well in 100 μL medium in 96-well black plates with half area transparent bottom (Greiner Bio-One). Each well was inoculated with 100 μL of viruses that were subjected to 10-fold serial dilution and infection was done in triplicate. After 48 h, viral infection was determined by a Firefly Luciferase Assay Kit (Biotium). Briefly, after removal of media, 20 μL of 1x Firefly Lysis Buffer were added to each well. After 5 m, 15 μL of Firefly Luciferase Assay Buffer 2.0 containing 0.3 μL of D-luciferin at 10 mg/ml were added to each well and luciferase activities were measured by Veritas Microplate luminometer (Turner Biosystem). Viral infectivity was calculated by normalizing luciferase activities with the amounts of p24Gag in viral inoculum.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!