Lsm 510 meta confocal scanning microscope

Manufactured by Zeiss

The LSM 510 Meta Confocal Scanning Microscope is a high-performance optical imaging system designed for advanced microscopy applications. It features a scanning laser that allows for the acquisition of high-resolution, three-dimensional images of samples. The LSM 510 Meta is capable of providing detailed information about the structure and composition of a wide range of materials and biological specimens.

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Lab products found in correlation

Anti megalin, by Santa Cruz Biotechnology (1 mentions) Cryostat, by Leica (1 mentions)

Close spelling variants of this product

LSM 510 META confocal scanning laser inverted microscope LSM 510 META NLO Two-Photon Laser Scanning Confocal Microscope System LSM 510 META Axioplan 2 confocal laser scanning microscope LSM 510 Meta argon/krypton laser scanning confocal microscope LSM 510 Meta confocal laser scanning microscope LSM 510 META confocal microscope LSM 510 confocal scanning microscope LSM 510 META confocal LSM 510 META microscope 510 Meta confocal microscope

2 protocols using lsm 510 meta confocal scanning microscope

Fluorescent Labeling and Localization of LCN2 in Mice

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LCN2 was fluorescently labelled with Alexa568-Succinimidyl Ester (Molecular Probes) by following previous protocols58 (link) and instructions from the manufacturer (https://tools.thermofisher.com/content/sfs/brochures/TR0031-Calc-FP-ratios.pdf) including clean up by gel filtration followed by microcon (10 K) washes. The molar ratios of Alexa dye to native, K3, K3Cys proteins were 0.98, 1.20 and 0.73. The proteins were introduced (80 μg for LCN2, 8-week old mice) and after 1 h, kidney, liver, heart and spleen were fixed in 4% PFA. Immunohistochemical staining of the kidney proximal tubules (Anti-Megalin, Santa Cruz; LTL, Vector Labs) collecting ducts (Anti-Tromal Ab, Developmental Studies Hybridoma; DSHB; Anti-AE1 Ab), Principal cells (Anti-AQP2), Intercalated cells (Anti-V-ATPase Ab) were visualized using Zeiss LSM 510 Meta Confocal Scanning Microscope. Immunoblots were assayed with anti-mouse LCN2 (R&D Systems) and anti-mouse Tf (Bethyl Laboratories, A90-129A).

Barasch J., Hollmen M., Deng R., Hod E.A., Rupert P.B., Abergel R.J., Allred B.E., Xu K., Darrah S.F., Tekabe Y., Perlstein A., Wax R., Bruck E., Stauber J., Corbin K.A., Buchen C., Slavkovich V., Graziano J., Spitalnik S.L., Bao G., Strong R.K, & Qiu A. (2016). Disposal of iron by a mutant form of lipocalin 2. Nature Communications, 7, 12973.

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Retinal Tissue Preparation and Immunohistochemistry

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Animals were euthanized by intraperitoneal administration of pentobarbital (120 mg/kg body weight administered intraperitoneally followed by intracardial administration), and the eyes were enucleated and fixed in 4% paraformaldehyde for 3 h at room temperature and then submerged in 20% sucrose in PBS (1×: 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2 mM KH₂PO₄, pH 7.4) overnight at 4 °C. Fixed eyes were embedded in optimal cutting temperature compound (OCT; Miles Diagnostics, Elkhart, IN) and frozen at −80 °C. Transverse 10 µm thick retinal sections were cut using a cryostat (Leica Biosystems, Buffalo Grove, IL) and used for immunohistochemical analysis. Sections were viewed with a Zeiss (Thornwood, NY) LSM 510 META confocal scanning microscope.

Phatak N.R., Stankowska D.L, & Krishnamoorthy R.R. (2016). Bcl-2, Bcl-xL, and p-AKT are involved in neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma. Molecular Vision, 22, 1048-1061.

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