All protocols were approved by the Animal Ethics Committee of The University of Tokyo. BALB/c-nu/nu male mice (5-weeks-old) were purchased from Sankyo Labo Service Corporation (Tokyo, Japan). Mice were anesthetized with avertin solution, which contained 2-, 2-, 2-tribromoethanol (Sigma-Aldrich) and tert-Amyl alcohol (Sigma-Aldrich) in Hank’s balanced salt solution (HBSS, Thermo Fisher Scientific). Renal subcapsule inoculation was described previously [45 (link), 46 (link)]. Kidneys were exposed on the back of anesthetized mice. Cells were reconstituted in 50 μl of HBSS. A syringe with a 29-gauge needle (Terumo, Tokyo, Japan) was inserted from the lower pole to the subrenal capsule. After inoculation, the incision was closed with a surgical clip.
Balb c nu nu male mice
Manufactured by Sankyo Labo Service
Sourced in Japan
BALB/c-nu/nu male mice are a strain of laboratory mice that are athymic, meaning they lack a functional thymus gland and are therefore immunodeficient. These mice are commonly used in biomedical research, particularly for the study of human cancers and the evaluation of new therapies.
Automatically generated - may contain errors
Lab products found in correlation
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (2 mentions)
D luciferin potassium salt, by Promega (2 mentions)
Propylene glycol, by Merck Group (2 mentions)
Indigo software, by Berthold Technologies (2 mentions)
D luciferin potassium solution, by Berthold Technologies (2 mentions)
Nightowl lb 981, by Berthold Technologies (2 mentions)
2 2 2 tribromoethanol, by Merck Group (1 mentions)
Tert amyl alcohol, by Merck Group (1 mentions)
4 protocols using balb c nu nu male mice
1
Mouse Xenograft Model for Cancer Research
2
Orthotopic Mouse Renal Tumor Model
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All experiments were approved by the Animal Ethics Committee of the University of Tokyo. The housing and handling conditions of the mice were consistent with the method above. Mouse renal orthotopic tumor models were conducted as previously described [22 (link), 29 (link)]. Briefly, BALB/c‐nu/nu male mice (5 weeks old) were purchased from Sankyo Labo Service Corporation (Tokyo, Japan). ccRCC cells (1.0 × 105) expressing Luc2 and mCherry were inoculated into the subrenal capsule of mice. For in vivo bioluminescence imaging, D‐luciferin potassium salt (200 mg·kg−1; Promega) was diluted in PBS and injected into mice intraperitoneally. For ex vivo bioluminescence imaging, the harvested kidneys and lungs were reacted with d ‐luciferin potassium solution for 10 min, and images were captured using NightOWL LB981 (Berthold Technologies, Bad Wildbad, Germany). Quantitative analysis was conducted using the IndiGO software (Berthold Technologies). Everolimus was reconstituted in saline solution (Otsuka, Tokyo, Japan) containing 5% Tween20 and 30% propylene glycol (Sigma‐Aldrich) and administered to mice (2.5 mg·kg−1) thrice weekly.
3
Orthotopic Renal Tumor Models for ccRCC
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Tumor‐forming ability of ccRCC cells in mice was analyzed using mouse renal orthotopic tumor models and bioluminescence imaging as previously described.11 All protocols were approved by the Animal Ethics Committee of the Graduate School of Medicine at The University of Tokyo. BALB/c‐nu/nu male mice (5‐weeks‐old) were purchased from Sankyo Labo Service Corporation (Tokyo, Japan). Firefly luciferase was introduced into ccRCC cells by infection of lentiviral vectors for bioluminescence imaging.12 The ccRCC cells were resuspended in HBSS (Thermo Fisher Scientific) and then orthotopically injected into mouse kidney (3 × 104 Caki‐1 cells or 3 × 104 OS‐RC‐2 cells in 50 μL per mouse, unless otherwise specified).
4
Mouse Renal Orthotopic Tumor Model
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All experiments were approved by the Animal Ethics Committee of the University of Tokyo. Mouse renal orthotopic tumor models were generated as previously described. [29] Brie y, BALB/c-nu/nu male mice (5weeks-old) were purchased from Sankyo Labo Service Corporation (Tokyo, Japan). ccRCC cells (1.0 × 10 5 ) expressing Luc2 and mCherry were inoculated into the subrenal capsule of mice. For in vivo bioluminescence imaging, D-luciferin potassium salt (200 mg/kg; Promega, Madison, WI) was diluted in PBS and injected into mice intra-peritoneally. For ex vivo bioluminescence imaging, the harvested kidneys and lungs were reacted with D-luciferin potassium solution for 10 min, and images were captured using Night OWL LB981 (Berthold Technologies, Bad Wildbad, Germany). Quantitative analysis was conducted using the IndiGO software (Berthold Technologies). Everolimus was reconstituted in saline solution (Otsuka, Tokyo, Japan) containing 5% Tween20 and 30% propylene glycol (Sigma-Aldrich) and administered to mice (2.5 mg/kg) thrice weekly.
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