Newborn (6 g, 24 h) and adult male Sprague-Dawley rats (250–280 g, 8 w), as well as BALB/c-nu mice (15 g, 4 w) were used in this study. All animal experiments were approved by Institutional Animal Care and Use Committee of Department of Laboratory Animal Science of Fudan University (Grant No. 20160780A176), and confirmed with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH publication no. 85 − 23, revised 1996). DATS, 3-aminopropyltriethoxysilane (APTES), LF, N-succinimidyl-S-acetylthioacetate (SATA), and Mal-PEG-NHS were obtained from Sigma-Aldrich (St Louis, MO). Other reagents were of analytical grade and used as purchased. All the solutions were prepared by Milli-Q water and deaerated with high-purity nitrogen.
Mal peg nhs
Manufactured by Merck Group
Mal-PEG-NHS is a functionalized polyethylene glycol (PEG) derivative used in bioconjugation applications. It contains a maleimide group for covalent attachment to thiol-containing biomolecules and an N-hydroxysuccinimide (NHS) ester group for coupling to amine-containing biomolecules. The PEG spacer provides increased solubility and flexibility to the conjugated molecules.
Automatically generated - may contain errors
2 protocols using mal peg nhs
1
Rat and Mouse Tissue Preparation
2
Optimized Bioconjugation Protocol for In Vivo Experiments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Newborn (6 g, 24 h) and adult male Sprague-Dawley rats (250-280 g, 8 w), as well as BALB/c-nu mice (15 g, 4 w) were used in this study. All animal experiments were approved by Institutional Animal Care and Use Committee of Department of Laboratory Animal Science of Fudan University (Grant No. 20160780A176), and con rmed with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH publication no. 85 - 23, revised 1996). DATS, 3aminopropyltriethoxysilane (APTES), LF, N-succinimidyl-S-acetylthioacetate (SATA), and Mal-PEG-NHS were obtained from Sigma-Aldrich (St Louis, MO). Other reagents were of analytical grade and used as purchased. All the solutions were prepared by Milli-Q water and deaerated with high-purity nitrogen.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!