Cytokeratin (AE1/AE3, Dako, 1:50) was used as an epithelial marker, while Mac387, CD163, CD68 and Ham56 (Dako, 1: 20) were used as macrophage markers, DC-SIGN was used as a dendritic cell marker, Langerin was used as a Langerhans cell marker, CD3 as a T cell marker, and CD20 as a B cell marker. Double immunofluorescence-FISH was performed as for ISH, with additional immunofluorescence detection performed by incubating primary antibodies overnight before the detection of signals with the appropriate fluorescent secondary antibodies. ISH signals were detected using anti-digoxigenin alkaline phosphatase conjugate and HNPP/Fast Red TR mix (Roche). The secondary antibody for immunofluorescence was generally anti-mouse fluorescein thiocyanate (FITC) (Invitrogen). Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) in anti-fade mounting medium (Vectashield, Vector Laboratories).
Ham56
Manufactured by Agilent Technologies
Sourced in United States
The Ham56 is a laboratory equipment product from Agilent Technologies. It is designed for sample preparation and processing tasks within a research or analytical laboratory setting. The core function of the Ham56 is to provide consistent and reliable sample handling capabilities to support various analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
Cd163, by Agilent Technologies (1 mentions)
Mac387, by Agilent Technologies (1 mentions)
Cytokeratin ae1 ae3, by Agilent Technologies (1 mentions)
Hnpp fast red tr mix, by Roche (1 mentions)
Vectashield, by Vector Laboratories (1 mentions)
β catenin, by Merck Group (1 mentions)
Biotinylated secondary antibody, by Vector Laboratories (1 mentions)
2 protocols using ham56
1
Multiplex Immunophenotyping of Tissue Samples
2
Immunohistochemical Analysis of Aortic Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immediately after surgical excision, aortas were immersed in fixative solution (4% paraformaldehyde) and embedded in paraffin, cut into 5 μm thick serial sections and placed on poly-L-lysine coated slides. Primary antibodies used were: Matrix Metalloproteinase-7, MMP-7 (Rabbit polyclonal; Abcam), Cambridge, UK β-CATENIN (Rabbit policlonal; Millipore) Bedford, MA, USA and HAM56 (Mouse monoclonal; Dako Glostrup, Denmark). Before incubation with primary antibodies, sections were washed and endogenous peroxidase activity was impressed with H2O2 and goat or horse serum block. Primary antibodies were detected using the avidin–biotin immunoperoxidase technique. Sections were incubated with an appropriate biotinylated secondary antibody (1:200; Vector Laboratories). Burlingame, CA, USA The chromogen used was 3,3′-diaminobenzidine. Haematoxylin was used for nuclear stain. Images were captured by Nikon Eclipse 80i microscope and digitized by Retiga 1300i Fast camera, magnification (×400).
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