Anti pink1 bc100 494 antibody

Cells were lysed in RIPA buffer and subjected to western blot analysis as described previously 51 (link). Anti-Cox2 (ab198286), anti-MFN2 (ab56889), anti-SDHB (ab14714), anti-P4HB (ab137110), anti-PMP70 (ab3421), anti-PGC-1α (ab191838), anti-TFAM (ab272885), and anti-NRF1 (ab175932) antibodies were purchased from Abcam (Cambridge, UK). An anti-LC3B (#L10382) antibody was purchased from Invitrogen (Waltham, MA, USA). Anti-ULK1 pS555 (#5869), anti-ULK1 pS317 (#37762), anti-ULK1 (#8054), and anti-GM130 (#12480) antibodies were purchased from Cell Signaling Technology (Danvers MA, USA). An anti-OPA1 (#612606) antibody was purchased from BD Biosciences (San Jose, CA, USA). An anti-PINK1 (#BC100-494) antibody was purchased from Novus Biologicals (Littleton, CO, USA). Anti-Tom20 (SC-11415) and anti-Actin (SC-47778) antibodies were purchased from Santa Cruz (Dallas, TX, USA). All western blot analyses were repeated three times. Band intensities were quantified using densitometry and ImageJ software (NIH).

Cells were lysed in RIPA buffer and subjected to western blot analysis as previously described. Anti-Mst1 (#3682), anti-Mst1 pT183 (#49332), anti-Mob1 (#3863), and anti-Mob1 pT35 (#8699) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Mst2 (ab52641), anti-SDHB (ab14714), anti-Cox4 (ab16056), and anti-BNIP3 (ab109362) antibodies were purchased from Abcam (Cambridge, UK). Anti-Mfn1 (SC-50330) and anti-Actin (SC-47778) antibodies were purchased from Santa Cruz (Dallas, TX, USA). An anti-PINK1 (#BC100-494) antibody was purchased from Novus Biologicals (Littleton, CO, USA). All of the western blot analyses were repeated at least three times. The band intensities were quantified using densitometry and ImageJ software (NIH).