We used the Cell Counting Kit‐8 (CCK‐8; Dojindo Molecular Technologies, Kumamoto, Japan) to evaluate the proliferation. Following transfection for 48 hours, H1838 and H1299 cells were resuspended and seeded into a 12‐well plate (104 cells per well) for 5 days. Twenty microliter MTT solutions (Sigma, Shanghai, China) were added into the culture with final concentration of 20 mg/mL for 2.5 hours. The solution was shaken for 5 minutes, leading to complete solubilization. The proliferation was evaluated each day for 5 days. Crystalline formazan was dissolved in 300 μL 5% sodium dodecyl sulfate solution for 24 hours, and the optical density at 490 nm was evaluated using the Spectramax M5 microplate monitor (Molecular Devices, CA, USA) following the manufacturer's instructions.
Spectramax m5 microplate monitor
Manufactured by Molecular Devices
Sourced in United States
The SpectraMax M5 microplate monitor is a versatile laboratory instrument designed for a wide range of absorbance, fluorescence, and luminescence-based assays. It is capable of performing rapid, accurate, and sensitive measurements across multiple detection modes using microplates.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using spectramax m5 microplate monitor
1
Cell Proliferation Evaluation with CCK-8
2
Cell Viability Quantification with CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Cell Counting Kit‐8 Toolkit (CCK‐8, Dojindo) was purchased to quantify the viability following the manufacturer's protocols. After cell culture for 36 hours, cells were re‐suspended and then loaded into a 24‐well plate (1 × 105 cells/well) for 5 days. Notably, 30 μL CCK‐8 solutions were used. The optical density at 450 nm was measured with a SpectraMax M5 microplate monitor (Molecular Devices).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!