Single cells suspensions were stained for 30 minutes in PBS (with 0.5% BSA and 2mM EDTA) with ‘cocktails’ of the following antibodies CD31-FITC clone 390, gp38-PE clone 8.1.1, CD45 PerCP/CY5.5 clone 30-F11 (from eBiosciences), EPCAM PE/Cy7 clone G8.8 (from Biolegend), Ki67-Alexa 647 and BrDU-Alexa647 (BD Pharmingen). Afterwards cells were washed twice, re-suspended and then analyzed using a Cyan-ADP (Dako) with forward/side scatter gates set to exclude non-viable cells. Data were analyzed with FlowJo software (Tree Star).
Cd45 percp cy5.5 clone 30 f11
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CD45 PerCP/CY5.5 clone 30-F11 is a monoclonal antibody that binds to the CD45 antigen. CD45 is a transmembrane protein tyrosine phosphatase that is expressed on the surface of all leukocytes. This antibody is conjugated with PerCP/CY5.5, a fluorescent dye, for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cyan adp, by Agilent Technologies (2 mentions)
Cd31 fitc clone 390, by Thermo Fisher Scientific (2 mentions)
Gp38 pe clone 8, by Thermo Fisher Scientific (2 mentions)
Epcam pe cy7 clone g8, by BioLegend (2 mentions)
Ki67 alexa fluor 647, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Cd90 fitc clone 5e10, by BD (1 mentions)
Hla dr fitc clone g46 6, by BD (1 mentions)
3 protocols using cd45 percp cy5.5 clone 30 f11
1
Multiparametric Flow Cytometry of Cells
2
Multiparametric Flow Cytometry Analysis
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Single-cell suspensions were stained for 30 min in PBS (with 0.5% BSA and 2 mM EDTA) with cocktails of the following Abs: CD31-FITC clone 390, gp38-PE clone 8.1.1, CD45 PerCP/Cy5.5 clone 30-F11 (from eBioscience), epithelial cell adhesion molecule (EPCAM) PE/Cy7 clone G8.8 (from BioLegend), Ki67–Alexa Fluor 647, and BrDU–Alexa Fluor 647 (BD Pharmingen). Afterwards cells were washed twice, resuspended, and then analyzed using a CyAn ADP (Dako) with forward/side scatter gates set to exclude nonviable cells. Data were analyzed with FlowJo software (Tree Star).
3
Flow Cytometric Phenotyping of hUCMSCs
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To identify the cell phenotype, 3rd generation cells were subjected to flow cytometric identification. The expression of surface markers of hUCMSCs was detected by flow cytometry. Cultured cells were resuspended in pre-chilled phosphate-buffered saline (PBS) at a concentration of 106 cells/ml and incubated with CD34-FITC Clone 4H11, CD45-PerCP-Cy5.5 Clone 30-F11 (all from EBioscience, Thermo Fisher Scientific, Waltham, MA, USA), HLA-DR-FITC Clone G46-6, CD105-APC Clone 266, CD90-FITC Clone 5E10 and CD73- PerCP-Cy™5.5 (all from BD Pharmingen, Franklin Lakes, NJ, USA), respectively, for 30 min at 4°C. Subsequently, the cells were washed twice with PBS and then flow cytometry (BD Biosciences, BD FACSCalibur) was used to detect surface markers in hUCMSC. Data were processed with FlowJo10 (FlowJo, LLC).
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