Log In Sign up
The largest database of trusted experimental protocols

G9545 is a laboratory equipment product. It is a general-purpose device used for various scientific applications. The core function of this product is to perform specific tasks related to research and analysis in a laboratory setting. No further details about the intended use or specific applications of this product are provided.

Automatically generated - may contain errors

Lab products found in correlation

Sc 53564, by Santa Cruz Biotechnology (2 mentions) Abt123, by Merck Group (2 mentions) Av44268, by Merck Group (2 mentions) Pa5 16545, by Thermo Fisher Scientific (1 mentions) A2547, by Thermo Fisher Scientific (1 mentions) A2547, by Merck Group (1 mentions)

2 protocols using g9545

1

Protein Expression Analysis of Fibrosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
From each AAA sample 25 μg of denatured protein was separated on 4–12% polyacrylamide by electrophoresis and then transferred to PVDF membrane. The membranes were then incubated with collagen type-1 (Millipore #ABT123, 1:2000), TGF-β1 (Sigma #AV44268, 1:1000), α-SMA (Sigma #A2547, 1:1000) and KFkB (Thermo-Fisher #PA516545). GAPDH (Sigma # G9545, 0.1 mg/ml) and Caveolin-1 (Santa Cruz # sc-53564, 1:1000) were used as loading controls. Membranes were treated with HRP-conjugated secondary antibody at room temperature for 1 h and evaluated with chemiluminescence. Blot band intensities were analyzed using ImageJ software.
Sastriques-Dunlop S., Elizondo-Benedetto S., Arif B., Meade R., Zaghloul M.S., Luehmann H., Heo G.S., English S.J., Liu Y, & Zayed M.A. (2024). Ketosis prevents abdominal aortic aneurysm rupture through C–C chemokine receptor type 2 downregulation and enhanced extracellular matrix balance. Scientific Reports, 14, 1438.
+ Open protocol
+ Expand
2

Protein Expression Analysis in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
From each AAA sample 25μg of denatured protein was separated on 4–12% polyacrylamide by electrophoresis and then transferred to PVDF membrane. The membranes were then incubated with collagen type-1 (Millipore #ABT123, 1:2000), TGF-β1 (Sigma #AV44268, 1:1000), and α-SMA (Sigma #A2547, 1:1000). GAPDH (Sigma # G9545, .1mg/ml) and Caveolin-1 (Santa Cruz # sc-53564, 1:1000) were used as loading controls. Membranes were treated with HRP-conjugated secondary antibody at room temperature for 1 hour and evaluated with chemiluminescence. Blot band intensities were analyzed using ImageJ software.
Zayed M., Sastriques-Dunlop S., Elizondo-Benedetto S., Arif B., Meade R., Zaghloul M., Luehmann H., Heo G., English S, & Liu Y. (2023). Ketosis Prevents Abdominal Aortic Aneurysm Rupture Through C-C Chemokine Receptor Type 2 Downregulation and Enhanced MMP Balance. Research Square.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!