For all experimental procedures (UV irradiation, sd-OCT, ffERG) the animals were anesthetized with an intraperitoneal injection of a mixture of xylazine (10 mg/kg xylazin 2 % Bernburg, Medistar, Ascheberg, Germany) and Ketamine (60 mg/kg Ketamin 10 %, CEVA, Düsseldorf, Germany), 1:10 diluted in saline. For longer anesthesia periods, 0.05 mL of the Ketamine-xylazine mixture were applied subcutaneously in the lumbar region every 20 minutes. The animals were kept on a heated plate at 37 °C during anesthesia to maintain body temperature. When the animals were euthanized at the end of the experiment, they were decapitated under deep Ketamine/xylazine anesthesia.
Ketamin
Manufactured by Ceva
Sourced in Germany, Belgium
Ketamin is a laboratory equipment used for various scientific and research applications. It is a general anesthetic that can be used to induce a trance-like state, provide pain relief, and for other purposes. The core function of Ketamin is to serve as a tool for scientific research and experimentation, without making any interpretations or extrapolations about its intended use.
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Lab products found in correlation
2 protocols using ketamin
1
Anesthesia Procedure for Animal Experiments
2
Tumor Resection and Metastasis Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tumor bearing mice were operated the 4th (D4) or 11th day (D11) after the end of the RT treatment or when tumor volume reaches 400mm³ under anesthesia with xylazine (75mg/kg, VMD, Anedonk, Belgium) and ketamin (10mg/kg, CEVA, Brussels, Belgium). Tumors were carefully removed and surgical resection included a margin of 3 mm of healthy tissue. Skin was suturated with 5-0 silk (Perma-hand, Ethicon). Tumor fragments were formol-fixed and paraffin-embedded or frozen in air phase of liquid nitrogen for protein and RNA extractions. For hypoxic area detection, mice were injected intraperitoneally with 100 mg/Kg of Pimonidazole (Hydroxyprobe-1, Chemicon) one hour before tumor resection. After surgery, mice were kept alive until D45 [18 (link)]. At sacrifice, lungs were formol-fixed and paraffin-embedded. Six lung sections of 5 μm, spaced by 10 sections of 5 μm, were immunostained with an antibody against human Ki67 as previously described [18 (link)]. Metastases were manually counted and classified according to their size (<10 cells, 10 to 50 cells, 50 to 100 cells, >100 cells).
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