Human mesenchymal stem cells (hMSCs) were obtained from Lonza Corporation (Basel, Switzerland). 4–6 passages of hMSCs were used in this study. The cell growth media of the hMSCs was Alpha-modified Eagle’s minimum essential medium (Invitrogen, Karlsbad, CA, USA) with 10% FBS (Invitrogen, CA, USA) and 1% antibiotics (Invitrogen, CA, USA). Osteogenic differentiation media of hMSCs were prepared by adding 10 mM β-glycerolphosphate (Sigma, St. Louis, MO, USA), 150 μg/mL ascorbic acid (Sigma, MO, USA), and 10 nM dexamethasone (Sigma, MO, USA) to cell growth media. All media were replaced every three days.
Alpha modified eagle s minimum essential medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alpha-modified Eagle's minimum essential medium is a cell culture medium formulated for the growth and maintenance of various cell lines. It provides the necessary nutrients, vitamins, and other components to support cell proliferation and survival in an in vitro environment.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hmscs, by Thermo Fisher Scientific (1 mentions)
Hmscs, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
β glycerol phosphate, by Merck Group (1 mentions)
Hmscs, by Lonza (1 mentions)
1640 medium, by Merck Group (1 mentions)
Ap1903, by MedChemExpress (1 mentions)
Ym155, by Cayman Chemical (1 mentions)
M csf, by BioLegend (1 mentions)
Polybrene, by Merck Group (1 mentions)
Gm csf, by Miltenyi Biotec (1 mentions)
2 protocols using alpha modified eagle s minimum essential medium
1
Osteogenic Differentiation of hMSCs
2
Engineered Melanoma and Immune Cells
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MO4, an OVA-expressing B16-F10 melanoma cell line of C57BL/6 origin, was cultured in Roswell Park Memorial Institute 1640 medium (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco). Murine iPSC-pMCs and GM-pMCs were established as described previously10 (link),13 (link) and cultured in alpha-modified Eagle’s minimum essential medium (Invitrogen) supplemented with 30 ng/mL GM-CSF (Miltenyi Biotec), 50 ng/mL M-CSF (BioLegend), and 20% FBS. HSV-TK- and iCasp9-expressing GM-pMCs or iPSC-pMCs were generated by transducing GM-pMCs with a lentiviral vector encoding a synthetic HSV-TK gene (psetz-hsv1tk, InvivoGen) or iCasp951 (link) co-expressed with truncated CD19 (GenBank: NM001770) in the presence of Polybrene (Sigma-Aldrich). All cell lines were tested and found to be free of mycoplasma contamination. GCV was purchased from Hoffmann-La Roche. AP1903 was purchased from MedChemExpress, and YM-155 was purchased from Cayman Chemical.
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