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Glutamax with hepes

Manufactured by Thermo Fisher Scientific
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GlutaMAX™with HEPES is a cell culture medium supplement that provides a stable source of L-glutamine. It is designed to help maintain cell growth and viability in various cell culture applications.

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Lab products found in correlation

Recombinant mouse m csf, by R&D Systems (2 mentions) Recombinant mouse gm csf, by R&D Systems (2 mentions) Neural dissociation kit, by Miltenyi Biotec (2 mentions) Tgf β1, by Miltenyi Biotec (2 mentions)

Close spelling variants of this product

GlutaMAX (10236 citations) HEPES (4332 citations) GlutaMAX + HEPES (4 citations)

2 protocols using glutamax with hepes

1

Isolation and Culture of Murine Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wildtype and C9 null mice were perfused with PBS/Heparin, their brains isolated and dissociated using the Neural dissociation kit (Miltenyi Biotech) and GentleMACS dissociator (NTDK Brain setting). The lysate was collected and passed through a 70μm strainer to obtain a single cell suspension. Next, myelin was removed from the cells by incubating with magnetic myelin beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotech) using the AutoMACs. Cells were then incubated with CD11b+ magnetic beads (CD11b (Microglia) MicroBeads, human and mouse, Miltenyi Biotech) and sorted using the AutoMACs. The CD11b+ cells were counted, plated (200K cells/well) and cultured in Microglia complete media containing DMEM/F-12, GlutaMAX™with HEPES (Invitrogen), 10% fetal bovine serum, 100μg/ml Penicillin/ Streptomycin/0.25μg/ml fungizone, with 10 ng/ml of the following growth factors: Recombinant mouse M-CSF, Recombinant mouse GM-CSF (R&D systems) and 50ng/ml TGF-β1 (Miltenyi Biotech) for 6 days before stimulation.
McCauley M.E., O’Rourke J.G., Yáñez A., Markman J.L., Ho R., Wang X., Chen S., Lall D., Jin M., Muhammad A.K., Bell S., Landeros J., Valencia V., Harms M., Arditi M., Jefferies C, & Baloh R.H. (2020). C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature, 585(7823), 96-101.
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2

Isolation and Culture of Murine Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wildtype and C9 null mice were perfused with PBS/Heparin, their brains isolated and dissociated using the Neural dissociation kit (Miltenyi Biotech) and GentleMACS dissociator (NTDK Brain setting). The lysate was collected and passed through a 70μm strainer to obtain a single cell suspension. Next, myelin was removed from the cells by incubating with magnetic myelin beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotech) using the AutoMACs. Cells were then incubated with CD11b+ magnetic beads (CD11b (Microglia) MicroBeads, human and mouse, Miltenyi Biotech) and sorted using the AutoMACs. The CD11b+ cells were counted, plated (200K cells/well) and cultured in Microglia complete media containing DMEM/F-12, GlutaMAX™with HEPES (Invitrogen), 10% fetal bovine serum, 100μg/ml Penicillin/ Streptomycin/0.25μg/ml fungizone, with 10 ng/ml of the following growth factors: Recombinant mouse M-CSF, Recombinant mouse GM-CSF (R&D systems) and 50ng/ml TGF-β1 (Miltenyi Biotech) for 6 days before stimulation.
McCauley M.E., O’Rourke J.G., Yáñez A., Markman J.L., Ho R., Wang X., Chen S., Lall D., Jin M., Muhammad A.K., Bell S., Landeros J., Valencia V., Harms M., Arditi M., Jefferies C, & Baloh R.H. (2020). C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature, 585(7823), 96-101.
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