Image quant las 500 chemiluminescence detection system

WB was performed as described [49 (link)]. Antibodies used were the following: CK1α, PARP, Mcl1, Ser45 β-catenin, Ser33/37/Thr41 β-catenin, total β-catenin, Ser473 AKT, total AKT (Cell signaling Technology, MA, USA); Mdm2 (Millipore, Italy), GAPDH (Ambion, USA); α-tubulin (Sigma-Aldrich, Italy); Bak (Merck, MA, USA); Bax, p21, p53 (Becton Dickinson, Italy); Caspase 3 (Enzo Life Science, UK). Images were acquired using the Image Quant LAS 500 chemiluminescence detection system (GE Healthcare, USA). Densitometric analysis was performed with Quantity One software (Bio-Rad, Italy).

WB was performed as described (27 (link)). Antibodies used were the following: CK1α, PARP, Mcl1, total β-catenin, Ser 473 AKT, total AKT, Ser 176/180, Ser 177/181 IKKα/α, total IKKα, IKKβ, Ser 536 NF-κB p65, Ser 652 CARD11, Tyr 223 BTK, total BTK, Ser 32 Ikbα, BCL10 (Cell signaling Technology, MA, USA); GAPDH (Ambion, USA), β-actin (Sigma-Aldrich, Italy); p21 (Becton Dickinson, Italy); Caspase 3 (Enzo Life Science, UK); total p65 (abcam, UK), DEPTOR (Millipore, Itlay); CARD11 and BTK for immunoprecipitation (Santa Cruz Biotechnology, Inc; Italy). Images were acquired using the Image Quant LAS 500 chemiluminescence detection system (GE Healthcare, USA).