Anti p62 sqstm

Manufactured by Abnova

Anti-p62/SQSTM is a laboratory product that detects the p62/SQSTM1 protein. p62/SQSTM1 is an important regulatory protein involved in various cellular processes. This product can be used for research purposes to analyze the expression and localization of the p62/SQSTM1 protein in different biological samples.

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2 protocols using anti p62 sqstm

Protein Extraction and Immunoblotting Analysis

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Cells and tissues were lysed in cell lysis buffer (#9803; Cell Signaling Technology) with complete protease inhibitor cocktail and phosSTOP phosphatase inhibitor cocktail (Roche). Protein extracts were resolved by SDS-PAGE, transferred to PVDF membranes and incubated with the following primary antibodies: anti-αSMA (#A2547; Sigma-Aldrich); anti-Mart1 (#sc-20032; Santa Cruz Biotechnology, Inc.); anti-GAPDH (#ab9485), anti-CTGF (#ab6992; Abcam); anti-AREG (#sc-74501Invitrogen); anti-TSC1 (#37-0400; Invitrogen); anti-LC3 (#0231-100), anti-p62/SQSTM (#H00008878-M01; Abnova); anti–β-actin (#A5441), anti–α-tubulin (#T5168; Sigma-Aldrich); anti-YAP (#4912), anti-YAP/TAZ (#8418), anti-Lamin A/C (#2032), anti-rpS6 (#2217), anti-phospho-rpS6(Ser240/244; #5364), anti-p4EBP1(Thr37/46; #2855), anti-pYAP (Ser127; #4911), anti-Lats1 (#3477), anti-TSC2 (#4308), and anti-Ub (#3936; Cell Signaling Technology). Nuclear and cytoplasmic protein extractions were performed with NE-PER kit (#78835; Thermo Fisher Scientific). Flag-tagged YAP protein complex was immunoprecipitated from cell lysates with anti-Flag antibody (#F1804; Sigma-Aldrich) and protein G–Sepharose (#17-0618-01; GE Healthcare). Immunocomplexes were washed in cell lysis buffer three times and analyzed by immunoblotting.

Liang N., Zhang C., Dill P., Panasyuk G., Pion D., Koka V., Gallazzini M., Olson E.N., Lam H., Henske E.P., Dong Z., Apte U., Pallet N., Johnson R.L., Terzi F., Kwiatkowski D.J., Scoazec J.Y., Martignoni G, & Pende M. (2014). Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. The Journal of Experimental Medicine, 211(11), 2249-2263.

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Comprehensive Protein Profiling Protocol

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The following primary antibodies were used: anti‐p62 (SQSTM) (Abnova), anti‐LAMP2 (Abcam), anti‐FGF21 (abcam), anti‐Bip (BD Biosciences), anti‐Gapdh (Santa Cruz), anti‐SREBP1c (Santa Cruz), anti‐SREBP2 (abcam), anti‐ATF6 (abcam), anti‐LC3 (Nanotools), anti‐Tom20 (SantaCruz), anti‐lipin1 (SantaCruz, sc‐376874). All other antibodies were from Cell Signalling.

Rashid T., Nemazanyy I., Paolini C., Tatsuta T., Crespin P., de Villeneuve D., Brodesser S., Benit P., Rustin P., Baraibar M.A., Agbulut O., Olivier A., Protasi F., Langer T., Chrast R., de Lonlay P., de Foucauld H., Blaauw B, & Pende M. (2018). Lipin1 deficiency causes sarcoplasmic reticulum stress and chaperone‐responsive myopathy. The EMBO Journal, 38(1), e99576.

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