Naringenin

Manufactured by Agilent Technologies

Naringenin is a laboratory standard compound used in research applications. It is a flavonoid compound extracted from various plant sources. Naringenin serves as a reference material for analytical and bioassay procedures.

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Lab products found in correlation

Plant dna extraction kit, by Tiangen Biotech (1 mentions) Bi3730xl sequenator, by Thermo Fisher Scientific (1 mentions) Dm4000m microscope, by Leica (1 mentions) Ptc200pcr, by Bio-Rad (1 mentions) Geldox xr gel imaging system, by Bio-Rad (1 mentions) Taq pcr mastermix, by Tiangen Biotech (1 mentions) Lc 20at hplc, by Shimadzu (1 mentions)

2 protocols using naringenin

Quantification of Citrus Flavonoids

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They were as follows: Retsch MM400 Ball Mill(Laichi co., Germany); PTC200PCR(BIO-Rad, American); GelDox XR gel imaging system (BIO-Rad, American); DYY-8C Electrophoretic apparatus (Beijing Liuyi Instrument, China); BI3730XL sequenator (Applied Bio-systems, American); KRQ-300P manual climatic box (ChongQing YinBe Test Instrument, China); Plant DNA Extraction Kit (Tiangen Biotech Co., China); 2×Taq PCR MasterMix (Tiangen Biotech Co., China); primer was compounded by Sangon Co., China. Shimadzu; LC-20AT HPLC (Shimadzu Co., Japan); DM4000M microscope (Leica, Germany); Agilent 8453 ultraviolet and visible spectrophotometer (Agilent Technologies Co., American); hesperidin (MUST-12041206); narirutin (MUST-14081915); hesperetin (MUST-14100914); naringenin (MUST-14112310), four reference substance were bought from Chengdu Mansite biological technology co. LTD, purity>98.0%. The other reagents were analytically pure.

Wang F., Chen L., Li F.Q., Liu S.J., Chen H.P, & Liu Y.P. (2018). The Increase of Flavonoids in Pericarpium Citri Reticulatae (PCR) Induced by Fungi Promotes the Increase of Antioxidant Activity. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 2506037.

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HPLC Quantification of Naringenin

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An Agilent 1100 series HPLC was used to measure the concentration of naringenin in supernatants. To separate naringenin from other chemicals in soils and amendments, we used a Gemini C18 reversed-phase column (Phenomenex Inc., Torrance, CA) with an injection volume of 15 μl and eluted with 0.25% H₃PO₄ and acetonitrile. The flow rate was 1 ml min⁻¹. During the first 6 min, we used 100% of a 0.25% H₃PO₄ mobile phase. During the next 4 min, acetonitrile was increased using a linear gradient to 12%, then 18% for the next 20 min, and finally 58% for 15 min. naringenin elution was monitored using absorbance at 280 nm, and concentrations were determined by comparing peak areas to standards prepared in methanol using naringenin from Cayman Chemical. Figure S1B shows the naringenin standard curve.

Del Valle I., Webster T.M., Cheng H.Y., Thies J.E., Kessler A., Miller M.K., Ball Z.T., MacKenzie K.R., Masiello C.A., Silberg J.J, & Lehmann J. (2020). Soil organic matter attenuates the efficacy of flavonoid-based plant-microbe communication. Science Advances, 6(5), eaax8254.

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