Purified genomic DNA was sheared into smaller fragments of random sizes by a focused ultrasonicator (Covaris, MA, USA). DNA fragments were electrophoresed in 0.8% General Purpose Agarose E-Gel (Invitrogen, CA, USA), by which fragments of desired lengths were obtained. With purified DNA fragments, we constructed the libraries with insertion sizes of 200 bp, 308 bp (three sets), 456 bp, 735 bp, 2 kb, 5 kb, 10 kb, Molecular Plant 9, 1066-1077, July 2016 ยช The Author 2016. 1073 and 20 kb (Supplemental Table 1) using the Illumina Paired-End DNA protocol. The libraries were then sequenced on an Illumina Genome HiSeq 2500 machine using the PE-100 protocol. Total RNA-seq libraries were prepared using TruSeq RNA Library Preparation Kit v2 (Illumina, CA, USA) according to the instructions. They were also sequenced on an Illumina Genome HiSeq 2500 machine using the PE-100 protocol.
Zhang J., Tian Y., Yan L., Zhang G., Wang X., Zeng Y., Zhang J., Ma X., Tan Y., Long N., Wang Y., Ma Y., He Y., Xue Y., Hao S., Yang S., Wang W., Zhang L., Dong Y., Chen W, & Sheng J. (2016). Genome of Plant Maca (Lepidium meyenii) Illuminates Genomic Basis for High-Altitude Adaptation in the Central Andes. Molecular plant, 9(7).
+ Open protocol