Mouse anti map2

Manufactured by Fortrea

Sourced in United States

Mouse anti-MAP2 is a primary antibody that recognizes the microtubule-associated protein 2 (MAP2). MAP2 is a cytoskeletal protein found in neurons and is important for the structure and function of neuronal dendrites. The mouse anti-MAP2 antibody can be used for the detection and localization of MAP2 in various applications such as immunohistochemistry, immunocytochemistry, and Western blotting.

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2 protocols using mouse anti map2

Immunofluorescence Staining Protocol for Neural Markers

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Immunofluorescence was performed on 4% paraformaldehyde (PFA)-fixed cells permeabilized with 0.25% Tween-20. Primary antibodies were mouse anti-Nestin (Millipore, Billerica, MA, USA), rabbit anti-Sox2 (Millipore), mouse anti-MAP2 (Covance, Princeton, NJ, USA), rabbit anti-GFAP (DAKO, Carpinteria, CA, USA), rabbit anti-Mushashi1 (Millipore), rabbit anti-Tuj1 (Millipore), mouse anti-NeuN (Millipore), mouse anti-Oct4 (Millipore), rabbit anti-Pax6 (Covance), rabbit anti-TUC4 (Millipore), mouse anti-Vglut1 (Synaptic Systems, Goettingen, Germany), mouse anti-BrdU (SBCT, Santa Cruz, CA, USA) and rabbit anti-Ki67 (Abcam, Cambridge, MA, USA). Species-specific Alexa-Fluor-labeled secondary antibodies was used for detection followed by mounting in Prolong gold anti-fade with DAPI (Life Technologies).

Yelamanchili S.V., Morsey B., Harrison E.B., Rennard D.A., Emanuel K., Thapa I., Bastola D.R, & Fox H.S. (2014). The evolutionary young miR-1290 favors mitotic exit and differentiation of human neural progenitors through altering the cell cycle proteins. Cell Death & Disease, 5(1), e982-.

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Immunolabeling of Embryonic Neural Cells

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Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously (Kim et al., 2009 ). The following primary antibodies were used: rabbit anti-MACF1 (Wu et al., 2011 (link)), rabbit anti-MACF1 (Santa Cruz), rabbit anti-phospho-MACF1 (Wu et al., 2011 (link)), chicken anti-nestin (Neuromics), mouse anti-MAP2 (Covance), rabbit anti-Tbr1 (Chemicon), rabbit anti-Cux1 (Santa Cruz), goat anti-Brn1 (Novus Biologicals), rabbit anti-acetyl-α-tubulin (Cell Signaling), mouse anti-α-tubulin (Sigma), and chicken anti-actin (Millipore). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies.

Ka M., Jung E.M., Mueller U, & Kim W.Y. (2014). MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. Developmental biology, 395(1), 4-18.

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