V158411 was synthesized according to the method described in [30 ] and prepared as a 20 mM DMSO stock in DMSO. Solid stocks were purchased from the indicated suppliers and prepared as concentrated stock solutions in the appropriate solvent: gemcitabine (Apin Chemicals Inc), 20 mM in H2O; cisplatin (Selleckchem), 3.33 mM in 1% NaCl in H2O; oxaliplatin (Tocris), 5 mM in H2O; carboplatin (Tocris), 25 mM in H2O; PF-477736 (Selleckchem), 20 mM in DMSO and AZD7762 (Axon Medchem), 20 mM in DMSO.
Azd7762
Manufactured by Axon Medchem
Sourced in Netherlands
AZD7762 is a small molecule that functions as a potent and selective inhibitor of checkpoint kinase 1 (Chk1). Chk1 is a serine/threonine protein kinase that plays a crucial role in the DNA damage response pathway, making it a potential target for cancer therapy.
Automatically generated - may contain errors
Lab products found in correlation
Pf 477736, by Selleck Chemicals (2 mentions)
Paclitaxel, by Chemietek (2 mentions)
Carboplatin, by Adipogen (2 mentions)
Olaparib, by Targetmol (2 mentions)
Mts assay system, by Promega (2 mentions)
Glomax discover, by Promega (2 mentions)
Cisplatin, by Merck Group (2 mentions)
Niraparib, by Axon Medchem (2 mentions)
Azd6738, by Axon Medchem (2 mentions)
Azd1775, by Axon Medchem (2 mentions)
Doxorubicin, by Merck Group (2 mentions)
Ku55933, by Axon Medchem (2 mentions)
Oxaliplatin, by Bio-Techne (1 mentions)
Cisplatin, by Selleck Chemicals (1 mentions)
Carboplatin, by Bio-Techne (1 mentions)
Prism 8, by GraphPad (1 mentions)
Verapamil, by Merck Group (1 mentions)
Prism 9, by GraphPad (1 mentions)
Metformin, by Merck Group (1 mentions)
Rotenone, by Merck Group (1 mentions)
4 protocols using azd7762
1
Synthesis and Preparation of Compounds
2
Inhibition of CHK1 with AZD7762 and PF477736
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The small molecular CHK1 inhibitor AZD7762 [26 (link)] was purchased from Axon Medchem (Groninberg, Netherlands) and CHK1 inhibitor PF477736 [27 (link)] was purchased through SelleckChem. The drugs were reconstituted in dimethyl sulfoxide (DMSO) and aliquots were stored at –20 °C. An equivalent amount of DMSO for the highest concentration of drugs were used as a vehicle-control for each experiment.
3
Cytotoxicity Evaluation of Anti-Cancer Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Olaparib was purchased from TargetMol; rucaparib, niraparib, KU55933 (ATM inhibitor), AZD6738 (ATR inhibitor), AZD7762 (Chk1 inhibitor) and AZD1775 (Wee1 inhibitor) from Axon Medchem; cisplatin from Sigma Adrich; carboplatin from Adipogen; paclitaxel from ChemieTek; doxorubicin and verapamil from Merck. All the drugs were dissolved in DMSO as stock solutions and diluted in medium just before treatment. For cytotoxicity experiments, cells were seeded at 1000 cells/mL and treated with different drug concentrations in 96-well plates 48 h after seeding. After five days, cell viability was examined with the MTS assay system (Promega) and absorbance was acquired using a plate reader (GloMax Discover, Promega). Drug concentrations inhibiting growth in 50% of the cells (IC50) were calculated for each cell line, with the interpolation method on Prism 8.3.0 (GraphPad Software).
4
Cytotoxicity assay of drug compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cisplatin, metformin and fenformin were purchased from Sigma-Aldrich (St. Louis, MO, USA); carboplatin from Adipogen (San Diego, CA, USA); paclitaxel from ChemieTek (Indianapolis, IN, USA); doxorubicin and rotenone from Merck; Yondelis (ET-743) from PharmaMar (Madrid, Spain); olaparib from TargetMol (Boston, MA, USA); oxaliplatin, rucaparib, niraparib, KU55933 (ATM inhibitor), AZD6738 (ATR inhibitor), AZD7762 (Chk1 inhibitor) and AZD1775 (Wee1 inhibitor) from Axon Medchem (Groningen, The Netherlands). All the drugs were dissolved in DMSO or water as stock solutions and diluted in medium just before treatment. For cytotoxicity experiments, cells were seeded at 1000–2000 cells/mL and treated with different drug concentrations in 96-well plates 48 h after seeding. After five days of treatment, cell viability was examined with the MTS assay system (Promega Corporation, Madison, WI, USA), and absorbance was acquired using a microplate reader (GloMax Discover, Promega Corporation). Drug concentrations inhibiting growth in 50% of the cells (IC50) were calculated for each cell line, with the interpolation method on Prism 9.5.1 (GraphPad Software, La Jolla, CA, USA). All the experiments were run at least three times in sestuplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!