Serum CRP was measured by turbidimetry (Abbott Architect ci16200; Abbott Diagnostics, Abbott Park, IL, USA), while full-blood WBCs with automated differential count were counted on a CELL-DYN 4000 haematology analyser (Abbott Diagnostics) and an Advia 120 Hematology System (Siemens Healthineers, Erlangen, Germany). Serum PCT was measured using a chemiluminescent assay (Advia Centaur BRAHMS PCT; Siemens Healthineers). Serum calprotectin was analysed with an ELISA (Calpro AS, Oslo, Norway) on an automated ELISA instrument (Dynex DS2 Automated ELISA System; Dynex Technologies Inc., Chantilly, VA, USA). Both plasma PTX3 and presepsin levels were analysed by commercially available sandwich ELISAs (R&D Systems, Minneapolis, MN, USA, and MyBioSource Inc., San Diego, CA, USA, respectively).
Advia centaur b r a h m s pct
Manufactured by Siemens
Sourced in Germany
The ADVIA Centaur B.R.A.H.M.S PCT is a laboratory equipment product manufactured by Siemens. It is used for the quantitative determination of procalcitonin (PCT) in human serum and plasma samples.
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Lab products found in correlation
3 protocols using advia centaur b r a h m s pct
1
Biomarkers for Sepsis Diagnosis
2
Serum Procalcitonin Measurement Protocol
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Serum procalcitonin levels were measured with fully automated chemiluminescent immunoassay using ADVIA Centaur B.R.A.H.M.S PCT (Siemens Healthcare Diagnostics, Berlin, Germany), according to the manufacturer’s instructions.
3
Febrile Episodes Biomarker Measurement
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For each febrile episode, blood samples were collected within 24 hours after development of fever to measure serum PCT and CRP levels. Serum PCT levels were measured with fully automated chemiluminescent immunoassay using ADVIA Centaur B.R.A.H.M.S PCT (Siemens Healthcare Diagnostics, Berlin, Germany) according to the manufacturer’s instructions. Serum CRP concentrations were measured using commercial turbidimetric immunoassay. Blood culture results were attained using a BACTEC FX automated blood culture system (Becton Dickinson, Sparks, MD, USA). When only one set of coagulase-negative staphylococci was detected, it was considered as contamination and a negative blood culture. Aspergillus antigen assay was performed using Platelia™ Aspergillus antigen immunoassay (Bio-Rad Laboratories, Marnes-la-Coquette, France).
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