Human visceral preadipocytes, preadipocyte growth supplement and preadipocyte differentiation supplement were purchased from ScienCell Research Laboratories (San Diego, California, USA). Fetal calf serum was purchased from Gibco (Carlsbad, California, USA), and penicillin‐streptomycin‐amphotericin B solution was purchased from Biological Industries (Cromwell, Connecticut, USA). Human visceral preadipocytes were maintained in Dulbecco's modified Eagle's medium/F12 medium containing 5% Fetal calf serum, preadipocyte growth supplement and penicillin‐streptomycin‐amphotericin B solution according to the manufacturer's protocol. Post‐confluent preadipocytes were replaced with medium containing pre‐adipocyte differentiation supplement and then continued onto differentiation for 5 days. The medium was changed every 3 days. VAP‐1 concentrations in cell culture supernatants were measured using the VAP‐1 human enzyme‐linked immunosorbent assay kit according to the manufacturer's protocol (Abcam, Cambridge, UK). Relative VAP‐1 levels were normalized to total cell counts.
Preadipocyte growth supplement
Manufactured by ScienCell
Sourced in United States
Preadipocyte growth supplement is a specialized media formulation designed to support the growth and proliferation of preadipocytes, which are precursor cells that can differentiate into mature adipocytes (fat cells). The supplement provides the necessary nutrients and growth factors to facilitate the expansion of preadipocyte populations in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin amphotericin b solution, by Sartorius (1 mentions)
Human visceral preadipocytes, by ScienCell (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
Penicillin streptomycin, by ScienCell (1 mentions)
Hpa v, by ScienCell (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ges 1, by American Type Culture Collection (1 mentions)
A549 cell, by American Type Culture Collection (1 mentions)
Preadipocyte differentiation medium, by ScienCell (1 mentions)
Hpa v cells, by ScienCell (1 mentions)
4 protocols using preadipocyte growth supplement
1
Visceral Preadipocyte Differentiation Assay
2
Differentiation of human preadipocytes
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HPA-v (cat. no. 7210; ScienCell Research Laboratories, Inc.) were isolated from human visceral fat tissue and cultured in preadipocyte medium (cat.no 7211; ScienCell Research Laboratories, Inc.) containing 5% fetal bovine serum, 100 IU/ml penicillin-streptomycin, and 1% preadipocyte growth supplement (cat.no. 7252; ScienCell Research Laboratories, Inc.). After reaching confluence, the HPA-v were induced to differentiate for 3 days in DMEM containing 0.1 mM 3-isobutyl-1-methylxanthine, 1 µM dexamethasone, and 5 µg/ml insulin. The differentiated HPA-v were then maintained in DMEM containing 5 µg/ml insulin for 6 days.
3
Adipogenic Differentiation of Human Preadipocytes
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HPA-v (ScienCell Research Laboratories, San Diego, CA, USA) were cultured in preadipocyte medium (PAM; ScienCell Research Laboratories) supplemented with 5% fetal bovine serum (Gibco), 1% preadipocyte growth supplement (ScienCell Research Laboratories), and 1% penicillin/streptomycin at 37°C in 5% CO2.
To induce differentiation, confluent HPA-v (day 0) were cultured in differentiation medium containing serum-free PAM supplemented with 250 nM insulin, 1 mM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, and 1 µM rosiglitazone. The culture medium was replaced after 4 days. Cells were then cultured in serum-free Dulbecco’s modified Eagle’s medium containing 250 nM insulin, which was replaced every 2 days until lipid accumulation was observed in the cells (day 10). HPA-v were harvested on days 0, 1, 4, 8, and 10 for further experiments.
To induce differentiation, confluent HPA-v (day 0) were cultured in differentiation medium containing serum-free PAM supplemented with 250 nM insulin, 1 mM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, and 1 µM rosiglitazone. The culture medium was replaced after 4 days. Cells were then cultured in serum-free Dulbecco’s modified Eagle’s medium containing 250 nM insulin, which was replaced every 2 days until lipid accumulation was observed in the cells (day 10). HPA-v were harvested on days 0, 1, 4, 8, and 10 for further experiments.
4
Isolation and Differentiation of Human Adipocyte Cells
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Human preadipocytes-viscereal (HPA-v) cells were obtained from ScienCell Research Laboratories (#7210, Carlsbad, CA, USA). Human non-smallcell lung cancer (NSCLC) A549 cells, human lung carcinoma H1299 cells, human gastric adenocarcinoma AGS cells, human non-tumorigenic bronchial epithelial cell NL20, and human normal gastric epithelial cell GES-1 were all acquired from ATCC (American Type Culture Collection). HPA-v cells were cultured in DMEM containing 5% fetal bovine serum (FBS), 1% preadipocyte growth supplement (#7252, ScienCell Research Laboratories), and 1% penicillin/streptomycin (P/S). Adipocyte differentiation was induced by incubating HPA-v cells with Preadipocyte Differentiation Medium (#7221, ScienCell Research Laboratories) according to the manufacturer’s protocol. Mature adipocytes and other cells were grown in DMEM supplemented with 10% FBS and 1% P/S. All cells were kept in an incubator with a humidified atmosphere containing 5% CO2 at 37°C.
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