Pf477736

Manufactured by Bio-Techne

PF477736 is a chemical compound produced by Bio-Techne. It is a small molecule inhibitor that targets a specific biological pathway or process. The core function of this product is to modulate the activity of a particular protein or signaling mechanism in in vitro laboratory experiments.

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Lab products found in correlation

Nu7441, by Bio-Techne (1 mentions) Ve 821, by Merck Group (1 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Azd7762, by Selleck Chemicals (1 mentions) Alamarblue cell viability reagent, by Thermo Fisher Scientific (1 mentions) Th 302, by Syngene (1 mentions) Ly2603618, by Selleck Chemicals (1 mentions) Goat anti rabbit hrp, by Merck Group (1 mentions) Goat anti mouse hrp, by Merck Group (1 mentions) Caspase glo 3 7 assay system, by Promega (1 mentions) Comet assay kit, by Bio-Techne (1 mentions) Radioimmunoprecipitation assay (ripa), by Merck Group (1 mentions) Sb218078, by Bio-Techne (1 mentions) Z vad fmk, by Promega (1 mentions) Nutlin 3, by Bio-Techne (1 mentions)

3 protocols using pf477736

Small Molecule Inhibitor Assay

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All small molecule inhibitors were dissolved in DMSO and diluted in DMEM with 0.5% serum to working concentrations and added to cells at indicated time points: NU7441 (Tocris), 2 μM; PF477736 (Tocris), 1μM; and ATR inhibitor IV (VE-821, EMD Millipore), 5 μM.

Heiser K., Nicholas C, & Garcea R.L. (2016). Activation of DNA Damage Repair Pathways by Murine Polyomavirus. Virology, 497, 346-356.

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Chk1 Inhibitor Sensitivity Assay

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TH-302 was from Syngene, AZD7762 and LY2603618 were from Selleck Chemicals, and PF477736 was from Tocris Bioscience. RIPA was from Sigma. Protease inhibitor cocktails were from Thermo Scientific. ChemiGlow substrate was from Proteinsimple. ECL reagent, Rad51 mAb, actin mAb, goat anti-rabbit HRP, goat anti-mouse HRP, and cell cycle reagents were from EMD Millipore. γH2AX mAb was from Epitomics. Antibodies against phospho-Histone H3, phospho-Cdc2 Y15 antibody, total Chk1, phospho-Chk1 (S296) were from Cell Signaling. Total Cdc2 p34 antibody was from Santa Cruz Biotechnology. FITC-conjugated goat anti-mouse secondary antibody and AlamarBlue cell viability reagent were from Life Technologies. Comet assay kit was from Trevigen. Caspase Glo 3/7 assay system was from Promega. Isogenic p53 proficient and deficient cell line pairs were from Horizon Discovery. All other cell lines were from ATCC.

Meng F., Bhupathi D., Sun J.D., Liu Q., Ahluwalia D., Wang Y., Matteucci M.D, & Hart C.P. (2015). Enhancement of hypoxia-activated prodrug TH-302 anti-tumor activity by Chk1 inhibition. BMC Cancer, 15, 422.

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Small Molecule Inhibitors in Cell Culture

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2 mM HU (Millipore Sigma, Cat#H8627) was prepared in culture medium
prior to each experiment. Nutlin-3 (Tocris, Cat#3984), Pifithrin-α (Focus
Biomolecules, Cat#10–2480), SB 218078 (Tocris, Cat#2560), PF477736
(Tocris, Cat#4277) and zVAD-FMK (Promega, Cat# G7231) stocks were prepared in
DMSO and kept at −20ºC until needed, when dilutions were prepared
in culture medium for cell treatment for final concentrations of 10 μM,
10 μM, 30 nM, 5nM and 50 μM, respectively.

Dwivedi V.K., Pardo-Pastor C., Droste R., Kong J.N., Tucker N., Denning D.P., Rosenblatt J, & Horvitz H.R. (2021). Replication stress promotes cell elimination by extrusion. Nature, 593(7860), 591-596.

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