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C57bl 6n male

Manufactured by Charles River Laboratories

The C57BL/6N male is a laboratory mouse strain commonly used in research. It is a widely utilized model for a variety of studies due to its genetic stability and well-characterized traits. The core function of this mouse strain is to serve as a standardized experimental subject for scientific investigations.

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5 protocols using c57bl 6n male

1

Behavioral Assays in Transgenic Mice

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All procedures were approved by the IACUC of NYULMC in compliance with the NIH guidelines for the care and use of laboratory animals. Mice were housed at 18–23°C with 40–60% humidity under a 12 h light–dark cycle (dark cycle, 10 p.m. to 10 a.m.), with food and water available ad libitum. Test animals were adult Drd1-cre (>8 weeks, RRID: MMRRC_030989-UCD) and DAT-ires-cre (>8 weeks, Jackson, RRID: IMSR_JAX:006660) mice. Ai9 mice (Jackson, RRID: IMSR_JAX:007909) were crossed with Drd1-cre mice for revealing D1R expression. Stimulus animals were adult C57BL/6N male and female mice, adult BALB/c male and female mice, or sexually experienced C57BL/6N and Swiss Webster males (aggressor) purchased from Charles River, and 3–7 days old pups were from test mice or wildtype C57BL/6N breeders. After surgery, all the animals are single housed. All experiments were performed during the dark cycle of the animals.
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2

Behavioral Assays in Transgenic Mice

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All procedures were approved by the IACUC of NYULMC in compliance with the NIH guidelines for the care and use of laboratory animals. Mice were housed at 18–23°C with 40–60% humidity under a 12 h light–dark cycle (dark cycle, 10 p.m. to 10 a.m.), with food and water available ad libitum. Test animals were adult Drd1-cre (>8 weeks, RRID: MMRRC_030989-UCD) and DAT-ires-cre (>8 weeks, Jackson, RRID: IMSR_JAX:006660) mice. Ai9 mice (Jackson, RRID: IMSR_JAX:007909) were crossed with Drd1-cre mice for revealing D1R expression. Stimulus animals were adult C57BL/6N male and female mice, adult BALB/c male and female mice, or sexually experienced C57BL/6N and Swiss Webster males (aggressor) purchased from Charles River, and 3–7 days old pups were from test mice or wildtype C57BL/6N breeders. After surgery, all the animals are single housed. All experiments were performed during the dark cycle of the animals.
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3

Neuroanatomical and Functional Mapping of Mouse Brain

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All procedures were approved by NYULMC IACUC in compliance with the NIH guidelines for Care and Use of Laboratory Animals. Adult mice 12–38 weeks of age were used for all studies. Mice were housed in 18–23°C with 40–60 % humidity under a 12 h light-dark cycle (10 p.m. to 10 a.m. light), with food and water available ad libitum. Animals used for CTB retrograde tracing and RNA-Seq experiments were 14–16 week old wild-type C57BL/6N male purchased from Charles River. Male Esr1–2A-Cre (14–38 weeks, Jackson Stock No. 017911), male Vgat-ires-Cre × Ai6 (12–16 weeks, Jackson stock No. 016962 and 007906)52 (link), male Vglut1-ires-Cre × Ai6 (12–16 weeks, Jackson stock No. 023527 and 007906) 53 (link) were used for functional, recording and histological experiments. Stimulus animals were adult C57BL/6N female and male or BALB/c male mice (> 8 weeks) purchased from Charles River. Stimulus animals were group housed. After surgery, all test animals were singly housed. All the experiments were performed during the dark cycle of the animals.
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4

Neuroanatomical and Functional Mapping of Mouse Brain

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All procedures were approved by NYULMC IACUC in compliance with the NIH guidelines for Care and Use of Laboratory Animals. Adult mice 12–38 weeks of age were used for all studies. Mice were housed in 18–23°C with 40–60 % humidity under a 12 h light-dark cycle (10 p.m. to 10 a.m. light), with food and water available ad libitum. Animals used for CTB retrograde tracing and RNA-Seq experiments were 14–16 week old wild-type C57BL/6N male purchased from Charles River. Male Esr1–2A-Cre (14–38 weeks, Jackson Stock No. 017911), male Vgat-ires-Cre × Ai6 (12–16 weeks, Jackson stock No. 016962 and 007906)52 (link), male Vglut1-ires-Cre × Ai6 (12–16 weeks, Jackson stock No. 023527 and 007906) 53 (link) were used for functional, recording and histological experiments. Stimulus animals were adult C57BL/6N female and male or BALB/c male mice (> 8 weeks) purchased from Charles River. Stimulus animals were group housed. After surgery, all test animals were singly housed. All the experiments were performed during the dark cycle of the animals.
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5

Developmental Mouse Intestine and Milk Sampling

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C57BL/6N male (n = 14) and female mice (n = 30) were purchased from Charles River Laboratory (Wilmington, MA) and fed standard chow diet (LabDiet 5001, Purina, Hayward, CA). Mice were housed in solid plastic hanging cages with a 12 h dark-light cycle under constant conditions. Mice were bred overnight and the appearance of a vaginal plug determined successful breeding with the day counted as gestational day (GD) 0. Newborn pups (eight per dam) were counted as postnatal day (PD) 0 and were weaned at PD21 to the chow diet fed ad libitum. Samples of small intestine, including duodenum and jejunum, were collected at regular intervals from GD13 to PD60. Milk was collected at PD0, 5, 10, 14, 16, 18, and 21. All animal procedures were approved by the University of California, Davis Institutional Animal Care and Use Committee.
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