Rmm4532

Manufactured by Thermo Fisher Scientific

The RMM4532 is a laboratory equipment model manufactured by Thermo Fisher Scientific. It is designed for general laboratory use. The core function of the RMM4532 is to perform routine measurements and analyses. Detailed specifications and intended use are not available in this unbiased, factual description.

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Lab products found in correlation

Fugene 6, by Promega (1 mentions) Rhs4743, by Thermo Fisher Scientific (1 mentions)

2 protocols using rmm4532

Lentiviral Vectors for Gene Knockdown and Overexpression

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The SF-LV-shRNA-GFP and SF-LV-shRNA-BFP plasmids were obtained from K.L. Rudolph's lab^{60 (link)}, scrambled shRNA was cloned from TRIPZ non-silencing shRNA (Open Biosystems, RHS4743) and Setd2 shRNAs were cloned from GIPZ mouse Setd2 shRNA (Open Biosystems, RMM4532). Lentiviruses (SF-LV-scramble-GFP and SF-LV-shSetd2-GFP) were generated by co-transfecting the packaging plasmids sPAX2 and MD2.G into 293T cells by Fugene 6 (Promega, E2691). The MLL-NRIP3 fusion gene was subcloned into the pMIG-IRES-GFP vector. For viral production, the pMIG-MLL-NRIP3 or empty control vector was co-transfected into the 293T packaging cell line with pCMV-VSVG and pKat. The lentivirus supernatants were collected 42 h after transfection, filtered through a 0.45-μm filter and used immediately to infect overnight-cultured bone marrow (BM) cells.

Zhu X., He F., Zeng H., Ling S., Chen A., Wang Y., Yan X., Wei W., Pang Y., Cheng H., Hua C., Zhang Y., Yang X., Lu X., Cao L., Hao L., Dong L., Zou W., Wu J., Li X., Zheng S., Yan J., Zhou J., Zhang L., Mi S., Wang X., Zhang L., Zou Y., Chen Y., Geng Z., Wang J., Zhou J., Liu X., Wang J., Yuan W., Huang G., Cheng T, & Wang Q.F. (2014). Identification of functional cooperative mutations of SETD2 in human acute leukemia. Nature genetics, 46(3), 287-293.

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Ectopic Expression of Ceacam1-L in G-2 Cells

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For the ectopic expression of Ceacam1-L in G-2 cells, Ceacam1 cDNA was extracted from the pcDNA-mCC1-4 via EcoRI-digestion and subsequently cloned into lentiviral vector LeGO-iG2 (using EcoRI restriction site), kindly provided by Dr. C. Stocking [81 (link)]. Sequences were confirmed by DNA sequencing (Seqlab, Germany). Production of lentiviral vectors and transduction of G-2 cells was performed as previously described [12 (link)]. Commercially available shRNA constructs against mouse Ceacam1 mRNA using the pGIPZ vectorsystem were purchased at Open Biosystems (# RMM4532). The production of the lentiviral particles was performed according to the manufacturer instructions. GFP positivity was used to enrich cells that received the vector via fluorescence-activated cell-sorting.

Florian W., Lenfert E., Gerstel D., von Ehrenstein L., Einhoff J., Schmidt G., Logsdon M., Brandner J., Tiegs G., Beauchemin N., Wagener C., Deppert W, & Horst A.K. (2016). CEACAM1 controls the EMT switch in murine mammary carcinoma in vitro and in vivo. Oncotarget, 7(39), 63730-63746.

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