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Chloroform chcl3

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Chloroform (CHCl3) is a high-purity, laboratory-grade solvent used in a variety of scientific applications. It is a clear, colorless liquid with a characteristic sweet, ethereal odor. Chloroform has a high density and is immiscible with water. It is commonly used as a solvent for various organic compounds, extractions, and other laboratory procedures.

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Lab products found in correlation

Milli q grade water, by Merck Group (2 mentions) Acetone, by Merck Group (2 mentions) Chlorobenzene, by Thermo Fisher Scientific (1 mentions) Anhydrous acetonitrile, by Merck Group (1 mentions) Diphenyl diselenide phse 2, by Merck Group (1 mentions) H 9500, by Hitachi (1 mentions) Dimethylformamide dmf, by Thermo Fisher Scientific (1 mentions) Rat tail collagen type 1, by Thermo Fisher Scientific (1 mentions) Pluronic f108, by Merck Group (1 mentions) Deuterated sodium hydroxide, by Merck Group (1 mentions) Normal rat chow feed, by Specialty Feeds (1 mentions) Deuterium oxide, by Merck Group (1 mentions) Dextromethorphan hydrobromide dxm, by Merck Group (1 mentions) Pierce 660 nm protein assay reagent, by Thermo Fisher Scientific (1 mentions) Potassium dihydrogen phosphate kh2po4, by Merck Group (1 mentions) Sodium azide, by Thermo Fisher Scientific (1 mentions) Phosphoric acid, by Thermo Fisher Scientific (1 mentions) Boric acid, by Thermo Fisher Scientific (1 mentions) Citric acid, by Thermo Fisher Scientific (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)

19 protocols using chloroform chcl3

1

Characterization of Perovskite Materials

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TQ1 (weight- and number-average molecular
weights, Mn = 76 kg mol–1 and PDI = 2.6), p(g42T-T) (Mn = 24 kg mol–1, PDI = 3.3), Mo(tfd-COCF3)3, and [Mo(tfd-COCF3)3]−Et4N+ and [Mo(tfd-COCF3)3]2–(Et4N+)2 were
prepared according to previously reported procedures.15 (link),27 (link),36 (link) Poly(ethylene oxide) (PEO, Mw = 200 kg mol–1), [TPA–Br3]+[SbCl6]− (Magic
Blue), dichloromethane (DCM, purity > 99.9%), and anhydrous acetonitrile
(AcN, purity > 99.8%) were purchased from Sigma-Aldrich. Chlorobenzene
(CB, purity > 99%) and chloroform (CHCl3, purity >99.8%)
were obtained from Fisher Scientific. All commercial solvents and
the Magic Blue dopant were used as received and without further purification.
Järsvall E., Biskup T., Zhang Y., Kroon R., Barlow S., Marder S.R, & Müller C. (2022). Double Doping of a Low-Ionization-Energy Polythiophene with a Molybdenum Dithiolene Complex. Chemistry of Materials, 34(12), 5673-5679.
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2

Structural and Thermal Characterization of Pyrrole-Based Nanocomposites

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Pyrrole (Himedia,
India) was distilled and stored at −5 °C
prior to use. Diphenyl diselenide [(PhSe)2, Sigma Aldrich,
India], anhydrous ferric chloride (FeCl3, Fisher Scientific,
India), and chloroform (CHCl3, Fisher Scientific, India)
used were of analytical reagent grade. The structural characterization
of samples was performed by X-ray diffraction (XRD) using a PW 3050
diffractometer (Cu Kα radiation, λ = 1.5418 Å). Fourier
transform infrared spectroscopy (FTIR) was performed on a PerkinElmer
RX-1 FTIR spectrophotometer in the range of 4000–500 cm–1. The thermal properties were measured by thermogravimetric
analysis (TGA) performed on a SEIKO TG/DTA 6200 machine at a heating
rate of 10 °C/min under N2. Dielectric studies of
synthesized samples were performed using a computer-controlled LCR
meter analyzer (Model Agilent 4285). Particle size and morphology
of synthesized nanocomposites have been characterized by scanning
transmission electron microscopy (STEM, Hitachi H-9500).
Jan T., Ahmad Rizvi M., Moosvi S.K., Najar M.H., Husain Mir S, & Peerzada G.M. (2021). A Switching-Type Positive Temperature Coefficient Behavior Exhibited by PPy/(PhSe)2 Nanocomposite Prepared by Chemical Oxidative Polymerization. ACS Omega, 6(11), 7413-7421.
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3

Modular PLGA-based Biomaterial Scaffold

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PLGA (80:20 L-lactide:glycolide) was purchased from Evonik Industries (Essen, Germany). Chloroform (CHCl3) and dimethylformamide (DMF) were purchased from Fisher Scientific (Waltham, MA, USA). N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinamide (NHS), 2-ethanesulfonic acid (MES Buffer), NaOH, and Pluronic® F-108 were purchased from Sigma Aldrich (St. Louis, MO, USA). Rat tail type I collagen and fibronectin, plasma, were purchased from Life Technologies (Carlsbad, California, USA).
Brown J.H., Das P., DiVito M.D., Ivancic D., Tan L.P, & Wertheim J.A. (2018). Nanofibrous PLGA Electrospun Scaffolds Modified with Type I Collagen Influence Hepatocyte Function and Support Viability In Vitro. Acta biomaterialia, 73, 217-227.
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4

NMR Metabolite Profiling for Rat Inflammation

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The NMR reagents used for measurements, namely deuterium oxide (D2O, 99.9%), deuterated methanol (CD3OD, 99.9%), deuterated sodium hydroxide (NaOD), trimethylsilylpropionic acid-d4 (TSP), dextromethorphan hydrobromide (C18H25NO.HBr, 99%), and potassium dihydrogen phosphate (KH2PO4), were purchased from Merck (Darmstadt, Germany). Lipopolysaccharide (LPS) injection derived from Escherichia coli 026: B6, sterile-filtered phosphate buffer saline (PBS), dextromethorphan hydrobromide (DXM), and sterile purified water were obtained from Sigma Aldrich (St. Louis, MO, USA). Absolute methanol (MeOH) and chloroform (CHCl3) were procured from Fisher Scientific UK (Leicester, UK). Ultrapure distilled water was prepared using a Milli-Q purification system. The normal rat chow feed was purchased from Specialty Feeds (Glen Forrest, Australia). The Pierce® 660 nm Protein Assay reagents were supplied by Thermo Scientific (Waltham, MA, USA), and bovine serum albumin (BSA, 99% pure grade) was purchased from Sigma Aldrich (St. Louis, MO, USA). The complete kit for cytokines quantification; G-series Rat Inflammation Array 1; catalog no: GSR-INF-1 was purchased from RayBiotech (Norcross, GA, USA).
Ahmad Azam A., Ismail I.S., Kumari Y., Shaikh M.F., Abas F, & Shaari K. (2020). The anti-neuroinflammatory effects of Clinacanthus nutans leaf extract on metabolism elucidated through 1H NMR in correlation with cytokines microarray. PLoS ONE, 15(9), e0238503.
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5

Enzymatic Degradation of P4HB Sutures

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Commercially available sutures of P4HB (Monomax® USP 1) were kindly supplied by B. Braun Surgical S.A.U. Weight and number average molecular weights of Monomax® samples were 215,000 and 68,000 g/mol, as determined by GPC.
Pseudomona cepacia and Rhizopus oryzae enzymes with a specific activity of 40.0 and 55.7 U/mg solid, respectively, were obtained from Sigma-Aldrich (Madrid, Spain). All reagents, citric acid, phosphoric acid, chloride acid, boric acid, sodium hydroxide, sodium azide, and chloroform (CHCl3) were supplied by Fisher Chemical (Hampton, NH, USA).
Keridou I., Franco L., del Valle L.J., Martínez J.C., Funk L., Turon P, & Puiggalí J. (2020). Microstructural Changes during Degradation of Biobased Poly(4-hydroxybutyrate) Sutures. Polymers, 12(9), 2024.
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6

Nerve Growth Factor Delivery in PC12 Cells

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Poly(ε-caprolactone) (Mw 80 kDa), glutaraldehyde, sorbitan monooleate (Span 80), hexamethyldisilazane (HMDS), sodium dodecyl sulfate (SDS), Dulbecco’s modified eagle’s medium (DMEM/F12) were purchased from Sigma, Singapore. Chloroform (CHCl3) was purchased from Fisher Scientific Company, Loughborough, UK. Micro BCA Protein Assay Kit was purchased from Thermo Scientific (Rockford, lL, USA). Sodium hydroxide (NaOH) was bought from Merck (Singapore). Nerve growth factor (NGF 7S) and ChemiKine Nerve Growth Factor Sandwich ELISA kit were purchased from Millipore, Singapore. Rat pheochromocytoma (PC12) cells in the adherent type [PC12 Adh (ATCC® CRL1721.1™)] were obtained from ATCC, Manassas, VA, USA, while fetal bovine serum (FBS), horse serum (HS), and trypsin/EDTA were purchased from Invitrogen/Gibco, Grand Island, NY, USA. [3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] was purchased from Promega, Madison, WI, USA. Alexa Fluor 594 Goat anti-Mouse IgG antibody was obtained from Invitrogen, Eugene, OR, USA.
Hu J., Tian L., Prabhakaran M.P., Ding X, & Ramakrishna S. (2016). Fabrication of Nerve Growth Factor Encapsulated Aligned Poly(ε-Caprolactone) Nanofibers and Their Assessment as a Potential Neural Tissue Engineering Scaffold. Polymers, 8(2), 54.
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7

Lipid Compound Characterization and Preparation

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Lipid compounds including 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethyleneglycol)-2000] [DSPE-PEG(2000)], 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol)-2000]-folate [DSPE-PEG (2000)-folate], 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPG), and cholesterol were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Free folate; PFP (29°C BP); and fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), and 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Chloroform (CHCl3) was purchased from Fisher Scientific (Waltham, MA, USA).
Liu J., Shang T., Wang F., Cao Y., Hao L., Ren J., Ran H., Wang Z., Li P, & Du Z. (2017). Low-intensity focused ultrasound (LIFU)-induced acoustic droplet vaporization in phase-transition perfluoropentane nanodroplets modified by folate for ultrasound molecular imaging. International Journal of Nanomedicine, 12, 911-923.
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8

Lipid Extraction and Identification Protocol

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The AMF was purchased from Anchor (Fonterra Co-operative Group Limited, New Zealand). 1,3 (d 5)-diheptadecanoyl-2-heptadecenoyl-glycerol (d 5-(17:0/17:1/17:0) TAG) was selected as the internal standard and was purchased from Avanti Polar Lipids (Birmingham, AL, USA). Methanol, hydrochloric acid (HCL), ethyl alcohol, n-Hexane, acetonitrile, isopropanol, chloroform (CHCl 3 ), formic acid, and ammonium formate were bought from Fisher Scientific (Pittsburgh, PA, USA). The standard mixture, comprised of 37 FAME, was bought from ANPEL Laboratory Technologies Inc. (Shanghai, China) .
Zhu H., Si X., Wang Y., Zhu P., Pang X., Wang X., Fauconnier M.L., Ju N., Zhang S, & Lv J. (2023). Fatty acid, triglyceride, and kinetic properties of milk fat fractions made by the combination of dry fractionation and short-path molecular distillation. Journal of dairy science, 106(10).
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9

Synthesis of metal nanoparticles using acetylacetonates

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Nickel(II) acetylacetonate (Ni(acac)2, 95%, Sigma-Aldrich), cobalt(II) acetylacetonate (Co(acac)2, 99%, Sigma-Aldrich), iron(III) acetylacetonate (Fe(acac)3, 97%, Sigma-Aldrich), iron(0) pentacarbonyl (Fe(CO)5, 99.99%, Sigma-Aldrich), dicobalt octylcarbonyl (Co2(CO)8, >90%, Sigma-Aldrich), tri-n-octylphosphine (TOP, (P(oct)3, 97% STREM), oleylamine (OAm, C18H37N, 70%, Sigma-Aldrich), octylether (C16H34O,
90%, TCI America), 1-octadecene (ODE, C18H36, 90% tech, Sigma-Aldrich), chloroform (CHCl3, 99%, Fisher
Scientific), ethanol (C2H5OH, 200 proof, Decon
Laboratories), and hexane (C6H14, >98.5%,
Fisher
Scientific) were used. Fe(CO)5, Co2(CO)8, TOP, and ODE were stored in a glovebox under argon. Oleylamine
was further dried over molecular sieves, while all other chemicals
were used as received.
Su’a T., Poli M.N, & Brock S.L. (2022). Homogeneous Nanoparticles of Multimetallic Phosphides via Precursor Tuning: Ternary and Quaternary M2P Phases (M = Fe, Co, Ni). ACS Nanoscience Au, 2(6), 503-519.
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10

Synthesis of Colloidal Nanocrystals

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Carbon disulfide (CS2, 99.9%, Sigma-Aldrich),
chloroform (CHCl3, 99.9%, Fisher), copper(II) chloride
dihydrate (CuCl2·2H2O, 99%, Sigma-Aldrich),
methylene blue (RICCA, surfactant analysis), 1-dodecanethiol (1-DDT,
98%, Aldrich), ethanol (EtOH, anhydrous, Decon), niobium(V) chloride
(NbCl5, 99%, Aldrich), selenium powder (Se, 99.99%, Aldrich),
1-octadecene (ODE, 90%, Aldrich), oleylamine (OLA, 70%, Aldrich),
and ammonium sulfide (aqueous (NH4)2S solution, 20–24%,
Alfa Aesar) were all used as received, without further purification.
Note. Standard Schlenk line techniques
were used for all syntheses. Inhalation of CS2 can cause
serious health problems, and therefore it is recommended to perform
all synthetic steps in a fume hood, wearing appropriate personal protective
equipment.
Chang C.Y., Prado-Rivera R., Liu M., Lai C.Y, & Radu D.R. (2022). Colloidal Synthesis and Photocatalytic Properties of Cu3NbS4 and Cu3NbSe4 Sulvanite Nanocrystals. ACS Nanoscience Au, 2(5), 440-447.
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