All cell lines were maintained in a humidified incubator at 37 °C with 5% CO2. PC9, HCC827, H3122, A549, A375, GR4, WZR12, PC9R, MGH134, MGH006, MGH1109, and MOLM13 were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MIA PaCa-2 and SW1573 were cultured in DMEM/F-12 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MGH119 were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. 293FT cells were cultured in DMEM high glucose medium with 10% FBS, 1% penicillin–streptomycin, 1% sodium pyruvate, 1% nonessential amino acids and 1% GlutaMax. All cell lines were purchased from American Type Culture Collection or Duke University Cell Culture Facility except for MGH lines, which were obtained from Dr. Aaron Hata. Cell lines were authenticated using the Promega PowerPlex 18D kit. Drugs were purchased from ApexBio (Osimertinib, Gefitinib, SCH772984, Ceritinib, Quizartinib, PLX4720, AMG510, Lorlatinib), Cayman Chemical (Olaparib), and SelleckChem (AZD0156, AZD1390, Q-VD-Oph, Cycloheximide).
Quizartinib
Manufactured by Cayman Chemical
Sourced in United States
Quizartinib is a small molecule inhibitor. It functions by targeting and inhibiting the FLT3 receptor tyrosine kinase.
Automatically generated - may contain errors
Lab products found in correlation
Gefitinib, by Cayman Chemical (2 mentions)
Powerplex 18d kit, by Promega (2 mentions)
Sch772984, by Cayman Chemical (2 mentions)
Azd0156, by Selleck Chemicals (2 mentions)
Osimertinib, by Apexbio (2 mentions)
Ceritinib, by Cayman Chemical (2 mentions)
Plx4720, by Cayman Chemical (2 mentions)
Amg510, by Cayman Chemical (2 mentions)
Olaparib, by Selleck Chemicals (2 mentions)
Q vd oph, by Selleck Chemicals (2 mentions)
Azd1390, by Selleck Chemicals (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Gilteritinib, by Merck Group (1 mentions)
Quizartinib, by Merck Group (1 mentions)
Pmscv flt3 itd y591f y919f, by Addgene (1 mentions)
Gilteritinib, by Cayman Chemical (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Ponatinib, by Cayman Chemical (1 mentions)
Cabozantinib, by Selleck Chemicals (1 mentions)
4 protocols using quizartinib
1
Cell Culture Maintenance and Compound Screening
2
K562 Differentiation into Megakaryocytes
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Human chronic myeloid leukemia (CML) in blast crisis cell line (K562) was procured from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained at 37°C, 5% CO2 in complete medium, containing Iscove’s Modified Dulbecco’s Medium (IMDM), 1X penicillin/streptomycin (Gibco), and supplemented with 10% (v/v) fetal bovine serum (FBS).
To differentiate K562 into Mk, cells were treated with Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, St. Louis, MO, USA), gilteritinib, quizartinib, dubermatinib (TP-0903), and midostaurin for 48–72 h as previously described (15 (link)). Where indicated, low-serum medium supplemented with 1% FBS and 1 μM imatinib were used.
gilteritinib, quizartinib, dubermatinib (TP-0903), and midostaurin were from Cayman chemical (Ann Arbor, MI, USA). All small molecules were dissolved in DMSO and store in −20°C. pMSCV-Flt3-ITD-Y591F/Y919F was procured from Addgene (Addgene.org). Flt3-ITD-Y591F/Y919F cassette was subcloned into lentiviral vector to produce viral particles using 293T cell.
To differentiate K562 into Mk, cells were treated with Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, St. Louis, MO, USA), gilteritinib, quizartinib, dubermatinib (TP-0903), and midostaurin for 48–72 h as previously described (15 (link)). Where indicated, low-serum medium supplemented with 1% FBS and 1 μM imatinib were used.
gilteritinib, quizartinib, dubermatinib (TP-0903), and midostaurin were from Cayman chemical (Ann Arbor, MI, USA). All small molecules were dissolved in DMSO and store in −20°C. pMSCV-Flt3-ITD-Y591F/Y919F was procured from Addgene (Addgene.org). Flt3-ITD-Y591F/Y919F cassette was subcloned into lentiviral vector to produce viral particles using 293T cell.
3
Cell Culture Maintenance and Compound Screening
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All cell lines were maintained in a humidified incubator at 37 °C with 5% CO2. PC9, HCC827, H3122, A549, A375, GR4, WZR12, PC9R, MGH134, MGH006, MGH1109, and MOLM13 were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MIA PaCa-2 and SW1573 were cultured in DMEM/F-12 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MGH119 were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. 293FT cells were cultured in DMEM high glucose medium with 10% FBS, 1% penicillin–streptomycin, 1% sodium pyruvate, 1% nonessential amino acids and 1% GlutaMax. All cell lines were purchased from American Type Culture Collection or Duke University Cell Culture Facility except for MGH lines, which were obtained from Dr. Aaron Hata. Cell lines were authenticated using the Promega PowerPlex 18D kit. Drugs were purchased from ApexBio (Osimertinib, Gefitinib, SCH772984, Ceritinib, Quizartinib, PLX4720, AMG510, Lorlatinib), Cayman Chemical (Olaparib), and SelleckChem (AZD0156, AZD1390, Q-VD-Oph, Cycloheximide).
4
Cell Culture and Drug Preparation
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All cell lines were purchased from DSMZ (Braunschweig, Germany) or ATCC (Manassas, VA, USA). The cell lines were regularly tested to exclude mycoplasma contamination and authenticated. All cell lines were grown at 37 °C and 5% CO2. A549 cells were cultured in DMEM medium (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA). All other cell lines were cultured in RPMI 1640 medium (Gibco™, Thermo Fisher Scientific). Both media were supplemented with 10% fetal bovine serum (FBS), 10 U/mL penicillin, 10 µg/mL streptomycin and 2 mM L-glutamine (all Gibco™, Thermo Fisher Scientific). Bortezomib (S1013; Selleck Chemicals, Houston, TX, USA), cabozantinib (S1119; Selleck Chemicals), cytarabine (PHR1787; Millipore Sigma, Burlington, MA, USA), gilteritinib (HY-12432; MedChemExpress, Monmouth Junction, NJ, USA), ispinesib (HY-50759; MedChemExpress), midostaurin (M1323; Millipore Sigma), ponatinib (11494; Cayman Chemical, Ann Arbor, MI, USA), quizartinib (17986; Cayman Chemical), venetoclax (HY-15531; MedChemExpress) and WS6 (S7442; Selleck Chemicals) were dissolved in dimethyl sulfoxide (DMSO; Carl Roth, Karlsruhe, Germany) and diluted further in culture medium immediately before use.
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