Benzo a pyrene bp

Alloxan (AL), streptozotocin (STZ) and benzo-(a)-pyrene (BP) were purchased from Sigma Aldrich (USA). Doxorubicin (DB) was purchased from Khandelwal Laboratories., (Thane, India). All culture media and additives were purchased from Sigma Aldrich Chemicals Pvt. Ltd., (Bangalore, India). BDNF and BDNF ELISA kits were purchased from Millipore, Massachusetts (USA), and lipoxin A4 (LXA4) ELISA kits were purchased from Oxford Biomedical Research (Michigan, USA).

Both RHT premade oral solution and RHF herbal mixture (composed of jack-in-the-pulpit tuber 9 g, common selfheal fruit-spike 12 g, seaweed 9 g, catclaw buttercup root 15 g, amorphophallus konjac 15 g, appendiculate cremastra pseudobulb 9 g, snakegourd peel 15 g, and dried tangerine peel 9 g, for each recipe) were obtained from the department of pharmacy, Longhua Hospital, affiliated to the Shanghai University of TCM, Shanghai, China. Benzo(a)pyrene (BP) and tricaprylin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Eight-week-old A/J mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA), fed, and maintained in the SIRF-West animal center at Washington University. The study was approved by Washington University’s Institutional Animal Care and Use Committee.