Nci h460

Fetal bovine serum was obtained from Sigma Aldrich. All the solvents, chemicals, and reagents were purchased from Hi Media laboratories (Mumbai). Cancer cell lines viz., MCF 7 (human breast cancer), HEP G2 (liver cancer), NCIH 460 (non-small cell lung cancer), and non-cancerous human kidney cell line, HEK293 were procured from the National Centre for Cell Sciences (NCCS), Pune.

The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India. The cell lines were cultured using a complete medium which includes, DMEM, 10% FBS, and 1% antibiotic antimycotic solution (penicillin, streptomycin, and amphotericin B). The cultured cell lines were incubated in a CO₂ incubator at an atmospheric temperature of 37°C and supplied with 5% CO₂.

Human non-small cell lung cancer cell lines (A549 and NCI-H460) and human monocytic THP-1 cell lines were procured from the National Centre for Cell Science (NCCS) Pune, India. Cells were cultured in RPMI1640 media supplemented with 10% FBS and 1% Penicillin-Streptomycin at 37°C in a humidified chamber with 5% CO2. MRC-5 human lung fibroblast cells were obtained from the ATCC, USA; and BEAS-2B human bronchial epithelial cells was a generous gift from Prof. Anita K. Verma. MRC-5 and BEAS-2B were cultured in EMEM and LHC-9 media, respectively, supplemented with 10% FBS and 1% Penicillin-Streptomycin solution at 37°C in a humidified chamber with 5% CO2.