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Varioskan flash spectral scanning multimode

Manufactured by Thermo Fisher Scientific
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The Varioskan Flash Spectral Scanning Multimode is a versatile laboratory instrument designed for spectral analysis. It is capable of performing absorbance, fluorescence, and luminescence measurements across a wide range of wavelengths. The instrument can be used to analyze various sample types, including microplates, cuvettes, and microvolumes.

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Varioskan Flash (1609 citations) Varioskan (275 citations) Varioskan Flash Spectral Scanning Multimode Reader (93 citations) Varioskan Flash Spectral (10 citations) Varioskan Flash spectral scanning (9 citations) Varioskan Flash Multimode (7 citations) Varioskan Flash spectral scanning multimode plate reader (4 citations) Varioskan Flash Spectral Scanning Multimode Microplate Reader (2 citations) Varioskan Flash-Spectral Scanning Multimode Microplate Reader 183 (2 citations)

3 protocols using varioskan flash spectral scanning multimode

1

Measuring Secreted PGE2 from Cultured Islets

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Media was collected from cultured islets after treatments, sieved through a 0.22 μm filter and diluted 3-fold in assay buffer. Secreted PGE2 levels were measured by ELISA, following the manufacturer’s instructions (R&D systems, Minneapolis, USA). Absorbance in each well was measured by using a Varioskan Flash Spectral Scanning Multimode (ThermoFisher Scientific) and serum PGE2 concentrations were calculated from the standard curve.
Martin Vázquez E., Cobo-Vuilleumier N., Araujo Legido R., Marín-Cañas S., Nola E., Dorronsoro A., López Bermudo L., Crespo A., Romero-Zerbo S.Y., García-Fernández M., Martin Montalvo A., Rojas A., Comaills V., Bérmudez-Silva F.J., Gannon M., Martin F., Eizirik D., Lorenzo P.I, & Gauthier B.R. (2022). NR5A2/LRH-1 regulates the PTGS2-PGE2-PTGER1 pathway contributing to pancreatic islet survival and function. iScience, 25(5), 104345.
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2

Quantifying Serum HMG20A Levels

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Secreted HMG20A levels were measured by ELISA, following the manufacturer's instructions (MyBioSource, San Diego, CA, USA). The sensitivity of the method was 1 ng/mL. Absorbance in each well was measured by using a Varioskan Flash Spectral Scanning Multimode (ThermoFisher Scientific) and serum HMG20A concentrations were calculated from the standard curve. Samples were run in duplicates.
Lorenzo P.I., Martin Vazquez E., López-Noriega L., Fuente-Martín E., Mellado-Gil J.M., Franco J.M., Cobo-Vuilleumier N., Guerrero Martínez J.A., Romero-Zerbo S.Y., Perez-Cabello J.A., Rivero Canalejo S., Campos-Caro A., Lachaud C.C., Crespo Barreda A., Aguilar-Diosdado M., García Fuentes E., Martin-Montalvo A., Álvarez Dolado M., Martin F., Rojo-Martinez G., Pozo D., Bérmudez-Silva F.J., Comaills V., Reyes J.C, & Gauthier B.R. (2021). The metabesity factor HMG20A potentiates astrocyte survival and reactive astrogliosis preserving neuronal integrity. Theranostics, 11(14), 6983-7004.
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3

Quantifying Serum HMG20A Levels

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Human serum levels of HMG20A were measured by ELISA, following the manufacturer's instructions (MyBioSource, San Diego, CA, USA). The sensitivity of the method was 1 ng/ml. Absorbance in each well was measured by using a Varioskan Flash Spectral Scanning Multimode (ThermoFisher Scientific) and serum HMG20A concentrations were calculated from the standard curve. Samples were run in duplicates.
Lorenzo P.I., Fuente-Martín E., Mellado‐Gil J.M., Guerrero-Martínez J.A., Cobo‐Vuilleumier N., Comaills V., Vázquez E.M., Romero‐Zerbo S.Y., Franco J.M., Pérez-Cabello J.A., Rivero S., Campos‐Caro A., Lachaud C.C., Aguilar‐Diosdado M., García‐Fuentes E., Martín‐Montalvo A., Álvarez‐Dolado M., Martı́n F., Rojo‐Martínez G., Pozo D., Bermúdez‐Silva F.J., Reyes J.C., & Gauthier B.R. (2021). The Metabesity Factor HMG20A Potentiates Astrocyte Survival and Reactivity Preserving Neuronal Integrity.
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