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L leucyl l leucine methyl ester

Manufactured by Merck Group

L-leucyl-L-leucine methyl ester is a chemical compound used as a laboratory reagent. It is a dipeptide consisting of two leucine amino acid residues. The compound is commonly used in research applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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2 protocols using l leucyl l leucine methyl ester

1

Antibody Reagents for Heparanase Research

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Anti-heparanase polyclonal antibody was purchased from Prospec (Rehovot, Israel). Anti-Hpa2 polyclonal (Ab 58) and monoclonal (20c5) antibodies have been described previously [4 (link)]. Anti-GRP78 (Bip), anti-CHOP and anti-ATF3 (ab254268) antibodies were purchased from Abcam (Cambridge, UK). Anti-AFT4 antibody was purchased from Santa Cruz Biotechnology (Santa Cruz CA); Anti-HIF1-α antibody (610958) was purchased from BD Transduction Laboratories. Anti-ATF3 polyclonal antibody has been described previously [28 (link)]. Anti-actin antibody, thapsigargin, tunicamycin, MG132, Cisplatin, PERK inhibitor GSK2606414, and L-leucyl-L-leucine methyl ester (LLOMe) were purchased from Sigma. Tissue arrays of cervical and hepatocellular carcinomas were purchased from US Biomax (Rockville, MD).
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2

Analyzing Digestive Enzyme Activities

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Collagenase from the bacteria Clostridium histolyticum was purchased from Serva (14,007). Enterokinase from the porcine intestine (E0885), Cholecystokinin (CCK) (C-9901), propidium iodide (p4170), and caerulein (C9026) were from Sigma Aldrich. Gly-Phe-β-napthylamide (GPN) was from Santa Cruz (sc-252858) and l-leucyl-l-leucine methyl ester was from Sigma Aldrich (BCCD0329). For enzyme kinetic measurements, the substrates for cathepsin B (AMC-Arg2, 4,004,789), cathepsin L (AMC-Phe-Arg, 4,003,379), and cathepsin C (H-Gly-Arg-AMC, 4,002,196) were purchased from Bachem. For trypsin activity measurement, the substrates were obtained from BioTrend (R110-Ile-Pro-Arg, 10,208) and Enzo Life Science (Boc-Gln-Ala-Arg-AMC, BML-P237-0005). Ketamine and Xylazine for mice anaesthetization were purchased from Selectavet. The lipase (LIPC, 03029590322) and amylase (Amyl Gen.2, 03183742122) measurement kits were obtained from Roche.
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