Mitf clone d5

Manufactured by Agilent Technologies

Sourced in Denmark

The MiTF (clone D5) is a monoclonal antibody product offered by Agilent Technologies for use in research applications. The antibody is designed to detect the Microphthalmia-associated transcription factor (MiTF), a protein that plays a key role in the development and function of certain cell types. The core function of this product is to provide researchers with a tool to identify and study the expression of MiTF in various biological samples.

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Lab products found in correlation

Sox10, by Cell Marque (1 mentions) Tfe3 clone mrq 37, by Roche (1 mentions) Desmin clone de r 11, by Roche (1 mentions) Cathepsink clone 3f9, by Abcam (1 mentions) Iview detection system, by Roche (1 mentions) Bcl2 clone 124, by Agilent Technologies (1 mentions)

Spelling variants of this product

Mouse monoclonal to MITF (clone D5, (4 citations) Anti-MITF (clone D5, (2 citations)

2 protocols using mitf clone d5

Immunohistochemical Profiling of Soft Tissue Neoplasms

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IHC was performed using an antibody panel consisting of TFE3 (clone MRQ-37; no dilution; Ventana Medical Systems, Tucson, AZ), HMB45 (clone HMB45; no dilution; Ventana), A103/MelanA (clone A103; no dilution; Ventana), SOX10 (polyclonal; 1:50 dilution; Cell Marque, Rocklin, CA), microphthalmia transcription factor (MiTF) (clone D5; 1:50 dilution; Dako, Carpinteria, CA), cathepsinK (clone 3F9; dilution 1:500; Abcam, Cambridge, MA), smooth muscle actin (SMA) (clone alpha-sm-1; 1:50 dilution; Vector Laboratories, Burlingame, CA), desmin (clone DE-R-11; no dilution; Ventana), h-caldesmon (clone E89; no dilution; Ventana). The Optiview or iView detection system (Ventana) was utilized for all antibodies with positive and negative controls as necessary.
Tumor cell immunoreactivity was semiquantitatively graded: 0 (negative); 1+ (1-25%); 2+ (26-50%); 3+ (51-75%); 4+ (>75%). Tumor cell immunoreactivity was also semiqualitatively graded as weak (W) or strong (S). For calculation of IHC totals, a minimum score of 1+ with weak or strong staining was considered positive with the exception of TFE3 which a 4+ and strong pattern was expected for a correlative TFE3 rearrangement.

Schoolmeester J.K., Dao L.N., Sukov W.R., Park K.J., Murali R., Hameed M.R, & Soslow R.A. (2015). TFE3 Translocation Associated Perivascular Epithelioid Cell Neoplasm (PEComa) of the Gynecologic Tract: Morphology, Immunophenotype, Differential Diagnosis. The American journal of surgical pathology, 39(3), 394-404.

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Immunohistochemical Analysis of Skin Biopsies

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Three transverse 4-lm tissue sections from each biopsy were taken from various levels and mounted on a Superfroste Plus Slide (Menzel-Gla ¨ser, Braunschweig, Germany). The slides were dried at 378C overnight. Immunohistochemical staining was performed on a Ventana Benchmarkt automated IHC stainer using the Ventana iViewe DAB detection kit (Ventanat Medical Systems, Tucson, AZ); subsequent manual counterstaining was performed with Meyers HTX. The primary antibodies were: monoclonal mouse antibody DNp63 (4A4) sc-8431, raised against amino acids 1-205 at the N-terminus of human DNp63 (1:200; Santa Cruz Biotechnologyt Inc., Dallas, TX); monoclonal mouse antibody MITF clone D5 (1:50; Dako, Glostrup, Denmark); and monoclonal mouse antibody Bcl-2 clone 124 (1:15; Dako). In addition, three tissue sections from each biopsy were stained with hematoxylin and eosin (H&E) and periodic acid-Schiff (eosin-PAS). Tissues known to express the antigen of interest were used as positive controls. As negative controls, skin biopsy sections omitting the primary antibodies from the staining procedure were used. For each molecular marker, all tissue sections from one patient were stained simultaneously to avoid influence from fluctuations in the procedure.

Fessé P., Qvarnström F., Nyman J., Hermansson I., Ahlgren J, & Turesson I. (2019). UV-Radiation Response Proteins Reveal Undifferentiated Cutaneous Interfollicular Melanocytes with Hyperradiosensitivity to Differentiation at 0.05 Gy Radiotherapy Dose Fractions. Radiation research, 191(1).

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