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4 protocols using linalool oxide

1

Characterization of Volatile Compounds

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β-Myrcene (≥90.0%) and cis-3-Hexenyl hexanoate (98%) were purchased from Macklin Biochemical Co. Ltd. (Shanghai, China). Benzylalcohol (≥99.5%), Linalool (98%), Nonanal (96%), α-Terpineol (>95.0%), Geraniol (≥99.0%), cis-Jasmone (98%), α-Farnesene (98%), and methyl palmitate (99%) were obtained from Aladdin Biochemical Co. Ltd. (Shanghai, China). Linalool oxide (pyranoid) and trans-Nerolidol were purchased from Yuanye Bio-Technology Co. Ltd. (Shanghai, China). n-Alkane mixtures of C7–C40 were supplied by Sigma-Aldrich Trading Co. Ltd. (Shanghai, China) and used to determine retention indices (RI). Ethyl decanoate was used as the internal standard and purchased from Aladdin Biochemical Co. Ltd. (Shanghai, China). Ultra-pure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA). Distilled water was purchased from the Hangzhou Wahaha Group Co. Ltd. (Hangzhou, China).
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2

Comprehensive Chemical Sourcing for Analytical Experiments

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Anhydrous
sodium sulfate (AR grade) was purchased from GHTECH (Shantou, Guangdong,
China); dichloromethane (AR grade) was ordered from PN-CHEM (Zhengzhou,
Henan, China). Tridecanol, 2-hexenal, 2-heptanol, (Z)-3-hexenyl acetate,
decanal, and benzyl nitrile were purchased from Sigma (St. Louis,
Missouri, USA); α-farnesene, cis-3-hexenyl hexanoate, 1-hexanol,
(Z)-3-hexenol, benzaldehyde, phytol acetate, ethyl decanoate, 3-octanol,
and guaiacol were obtained from Macklin (Shanghai, China); β-ocimene,
α-copaene, germacrene D, and neophytadiene were purchased from
J&K Chemicals (Shanghai, China); cis-jasmone, caffeine, and (E)-2-hexenol
were obtained from ChemFaces (Wuhan, China); indole was purchased
from Solarbio (Beijing, China); benzyl alcohol, linalool, geraniol,
nerol, nerolidol, α-terpinene, α-humulene, hexadecanoic
acid methyl ester, phytol, phenylacetaldehyde, α-terpineol,
2-phenylethanol, 1-octen-3-ol, δ-cadinene, caryophyllene oxide,
neral, methyl salicylate, and linalool oxide were purchased from Yuanye
Bio-Technology Co. (Shanghai, China). The alkane standard (C6–C44) was purchased from AccuStandard (New Haven,
CT, USA).
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3

Comprehensive Phytochemical Analysis of Aurantiacus, Latifolius and Thunbergii

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Acetonitrile and methanol of HPLC grade were purchased from Merck (Darmstadt, Germany). The standard products such as salidroside, verbascoside, oleanolic acid and ursolic acid were obtained from National Institutes for Food and Drug Control (Beijing, China). L (-)-2-Octanol (as the internal standard), linalool, linalool oxide, geraniol, α-ionone and β-ionone were bought from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Petroleum ether with the boiling range 80-120 To narrate bellows conveniently, they were tentatively called as "Aurantiacus", "Latifolius" and "Thunbergii".
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4

Phytochemical Analysis and Bioactivity Assay

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Acetonitrile and methanol (HPLC-grade) were purchased from Merck (Darmstadt, Germany). Standard products such as salidroside, acteoside, oleanolic acid, and ursolic acid were obtained from the National Institutes for Food and Drug Control (Beijing, China). L (-)-2-Octanol (as internal standard), linalool, linalool oxide, geraniol, α-ionone, and β-ionone were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Light petroleum, boiling range of 80 to 120 °C, was purchased from J&K Chemical, Ltd. (Beijing, China). Water of HPLC grade was purified by a reverse osmosis system (Millipore, Ireland). All the reagents of HPLC grade were filtered across a 0.45 µm membrane before the experiment. Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-y 1)-2,5-diphenyltetrazolium bromide (MTT), trypan, concanavalin A (Con A), penicillin G, and streptomycin sulfate were obtained from Sigma Chemical Co. (St. Louis, MO, USA), and Gibco 1640 medium and fetal bovine serum (FBS) from Gibco Invitrogen Corp. (San Diego, CA, USA). Other reagents and chemicals were of analytical grade.
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