Erk1 erk2

Manufactured by Santa Cruz Biotechnology

ERK1/ERK2 is a product offered by Santa Cruz Biotechnology, which is a laboratory equipment used for protein detection and analysis. It is a pair of enzymes, ERK1 and ERK2, that play a role in cellular signaling pathways.

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Lab products found in correlation

Anti p mek1 2, by Cell Signaling Technology (1 mentions) Anti craf, by BD (1 mentions) Anti grb2, by BD (1 mentions) Anti β actin mab, by Merck Group (1 mentions) Anti tubulin, by Fortrea (1 mentions) Phosphor akt, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Alexa conjugated fluorescent secondary antibodies, by Thermo Fisher Scientific (1 mentions)

2 protocols using erk1 erk2

Antibody Reagents for Erk, Raf, Ras, and Grb2 Signaling

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Rabbit polyclonal antibodies to ERK (ERK1/ERK2) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phospho-ERK protein, anti-MEK (MEK1/MEK2), and anti-p-MEK1/2 were purchased from Cell Signaling Technology (Beverly, MA), and anti-tubulin was from Covance (Princeton, NJ). Monoclonal antibodies Anti-C-RAF, anti-pan-Ras, and anti-Grb2 were obtained from BD Transduction Laboratories (Franklin Lakes, NJ), whereas rabbit polyclonal anti-Shoc2 was produced and purified in the lab. Anti-HA and AU5 monoclonal antibodies (mAb) were purchased from Berkeley Antibody Company (Berkeley, CA). Anti β-actin mAb, and recombinant human basic fibroblast growth factor (bFGF), nerve growth factor (NGF), and epidermal growth factor (EGF) were from Sigma-Aldrich (St. Louis, MO). Anti-mouse and rabbit HRP-conjugated mAb were from GE Healthcare Bio-Sciences (Piscataway, NJ).

Leon G., Sanchez-Ruiloba L., Perez-Rodriguez A., Gragera T., Martinez N., Hernandez S., Anta B., Calero O., Garcia-Dominguez C.A., Dura L.M., Peña-Jimenez D., Castro J., Zarich N., Sanchez-Gomez P., Calero M., Iglesias T., Oliva J.L, & Rojas J.M. (2014). Shoc2/Sur8 Protein Regulates Neurite Outgrowth. PLoS ONE, 9(12), e114837.

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TrkB Receptor Signaling in Neurons

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Human recombinant BDNF and NT4 were obtained from PeproTech (Rocky Hill, NJ). Immunoprecipitations and immunocytochemistry were performed with the following antibodies: rabbit anti TrkB from Upstate or goat from R&D; rabbit SHP2 from Santa Cruz (SC-280); Flag and tubulin antibodies were purchased from Sigma. The following antibodies: TrkB, SHP2, EEA1 and Grb2 were purchased from BD Biosciences. The phosphotyrosine PY99, actin-HRP, Erk1, Erk2 were from Santa Cruz; tubulin from Sigma. Phospho-Erk, phosphor-Akt and Akt were from Cell Signaling. Alexa conjugated fluorescent secondary antibodies were from Molecular Probes. All other compounds were from Sigma-Aldrich. All reagents used to prepare primary neuronal cultures were purchased from Invitrogen, except glucose that was from Sigma.

Proenca C.C., Song M, & Lee F.S. (2016). Differential effects of BDNF and neurotrophin 4 (NT4) on endocytic sorting of TrkB receptors. Journal of neurochemistry, 138(3), 397-406.

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