EDTA-anticoagulated peripheral blood from healthy donors was used to isolate neutrophils using EasySep Direct Human Neutrophil Isolation Kit (StemCell Technologies, USA) following the manufacturer’s instructions. Erythrocytes were lysed with red blood cell lysis buffer and washed with PBS. Subsequently, purity of the human neutrophils was evaluated by FACS using antibodies against Ly-6G/Ly-6C (1:500 dilution, Ly-6G/Ly-6C monoclonal antibody, PE, Thermo, USA) and CD11b (1:500 dilution, CD11b monoclonal antibody, FITC, Thermo, USA). Neutrophil purity was >93% (Supplementary Fig. 4 ).
Cd11b monoclonal antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CD11b monoclonal antibody is a laboratory reagent used for the identification and analysis of cells expressing the CD11b antigen, a cell surface molecule involved in cell-cell interaction and adhesion. This antibody can be used in various immunological techniques, such as flow cytometry, to detect and characterize cells of interest.
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Lab products found in correlation
Easysep direct human neutrophil isolation kit, by STEMCELL (1 mentions)
Methacrylic anhydride, by Merck Group (1 mentions)
Acryloyl chloride, by Merck Group (1 mentions)
Lithium phenyl 2 4 6 trimethylbenzoylphosphinate lap, by Merck Group (1 mentions)
Polycaprolactone triol, by Merck Group (1 mentions)
Phorbol ester phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)
Pluronic f 127 f 127, by Merck Group (1 mentions)
Anti mannose receptor antibody cd206, by Abcam (1 mentions)
2 protocols using cd11b monoclonal antibody
1
Isolation of Human Neutrophils
2
Multifunctional Hydrogel for Tissue Regeneration
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3,3,-glycidyl methacrylate (GMA), pluronic-F-127 (F127, weight average molecular weight, ~12.6 kDa), acryloyl chloride, lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), polycaprolactone triol [number-average molecular weight (Mn), ~900], methacrylic anhydride (MMA), tannins (TA), and phorbol ester [phorbol 12-myristate 13-acetate (PMA)] were purchased from Sigma-Aldrich (USA). Water-soluble SF was purchased from Suzhou Yongqinquan Intelligent Equipment Co., Ltd. (China). PDMS was purchased from Dow Chemical (USA). DEX, PNIPAm (Mn, ~40,000) and 5-FU were purchased from Shanghai Aladdin Biochemical Technology Co. Ltd. (China). Cell counting kit-8 (CCK-8) and apoptosis detection kit were obtained from Dojindo Chemical Technology Co., Ltd. (Shanghai). Cell viability/cytotoxicity kit was purchased from Beijing Solarbio Science & Technology Co., Ltd. (China). Tunel staining kit was purchased from Wuhan Servicebio Biotechnology Co., Ltd. (China). For antibody information, the following antibodies were used: anti-mannose receptor antibody (CD206, Abcam), anticytokeratin13 antibody (CK13, Abcam), anti–TNF-α antibody (TNF-α, Abcam), anti–IL-1 beta antibody (TNF-α, Abcam), anti-CK5 antibodies (Bioss), CD11b monoclonal antibody (Thermo Fisher Scientific), and anti-Ki67 antibody (Ki67, Abcam).
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